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Genotyping-by-sequencing markers facilitate the identification of quantitative trait loci controlling resistance to Penicillium expansum in Malus sieversii
Year:
2017
Source of publication :
PLoS ONE
Authors :
דרובי, סמיר
;
.
לוין, ילנה
;
.
Volume :
12
Co-Authors:
Norelli, J.L., Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Kearneysville, WV, United States
Wisniewski, M., Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Kearneysville, WV, United States
Fazio, G., Plant Genetic Resources Research, Agricultural Research Service, United States Department of Agriculture, Geneva, NY, United States
Burchard, E., Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Kearneysville, WV, United States
Gutierrez, B., Plant Genetic Resources Research, Agricultural Research Service, United States Department of Agriculture, Geneva, NY, United States
Levin, E., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Droby, S., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:
Blue mold caused by Penicillium expansum is the most important postharvest disease of apple worldwide and results in significant financial losses. There are no defined sources of resistance to blue mold in domesticated apple. However, resistance has been described in wild Malus sieversii accessions, including plant introduction (PI)613981. The objective of the present study was to identify the genetic loci controlling resistance to blue mold in this accession. We describe the first quantitative trait loci (QTL) reported in the Rosaceae tribe Maleae conditioning resistance to P. expansum on genetic linkage group 3 (qM-Pe3.1) and linkage group 10 (qM-Pe10.1). These loci were identified in a M.× domestica 'Royal Gala' X M. sieversii PI613981 family (GMAL4593) based on blue mold lesion diameter seven days post-inoculation in mature, wounded apple fruit inoculated with P. expansum. Phenotypic analyses were conducted in 169 progeny over a four year period. PI613981 was the source of the resistance allele for qM-Pe3.1, a QTL with a major effect on blue mold resistance, accounting for 27.5% of the experimental variability. The QTL mapped from 67.3 to 74 cM on linkage group 3 of the GMAL4593 genetic linkage map. qM-Pe10.1 mapped from 73.6 to 81.8 cM on linkage group 10. It had less of an effect on resistance, accounting for 14% of the experimental variation. 'Royal Gala' was the primary contributor to the resistance effect of this QTL. However, resistance-associated alleles in both parents appeared to contribute to the least square mean blue mold lesion diameter in an additive manner at qM-Pe10.1. A GMAL4593 genetic linkage map composed of simple sequence repeats and 'Golden Delicious' single nucleotide polymorphism markers was able to detect qM-Pe10.1, but failed to detect qM-Pe3.1. The subsequent addition of genotyping-by-sequencing markers to the linkage map provided better coverage of the PI613981 genome on linkage group 3 and facilitated discovery of qM-Pe3.1. A DNA test for qM-Pe3.1 has been developed and is currently being evaluated for its ability to predict blue mold resistance in progeny segregating for qMPe3.1. Due to the long juvenility of apple, the availability of a DNA test to screen for the presence of qM-Pe3.1 at the seedling stage will greatly improve efficiency of breeding apple for blue mold resistance. © This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Note:
Related Files :
chromosome mapping
disease resistance
genetic markers
Genetics
Microbiology
Penicillium
phenotype
עוד תגיות
תוכן קשור
More details
DOI :
10.1371/journal.pone.0172949
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32778
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:12
Scientific Publication
Genotyping-by-sequencing markers facilitate the identification of quantitative trait loci controlling resistance to Penicillium expansum in Malus sieversii
12
Norelli, J.L., Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Kearneysville, WV, United States
Wisniewski, M., Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Kearneysville, WV, United States
Fazio, G., Plant Genetic Resources Research, Agricultural Research Service, United States Department of Agriculture, Geneva, NY, United States
Burchard, E., Appalachian Fruit Research Station, Agricultural Research Service, United States Department of Agriculture, Kearneysville, WV, United States
Gutierrez, B., Plant Genetic Resources Research, Agricultural Research Service, United States Department of Agriculture, Geneva, NY, United States
Levin, E., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Droby, S., Department of Postharvest Science, Agricultural Research Organization, Volcani Center, Bet Dagan, Israel
Genotyping-by-sequencing markers facilitate the identification of quantitative trait loci controlling resistance to Penicillium expansum in Malus sieversii
Blue mold caused by Penicillium expansum is the most important postharvest disease of apple worldwide and results in significant financial losses. There are no defined sources of resistance to blue mold in domesticated apple. However, resistance has been described in wild Malus sieversii accessions, including plant introduction (PI)613981. The objective of the present study was to identify the genetic loci controlling resistance to blue mold in this accession. We describe the first quantitative trait loci (QTL) reported in the Rosaceae tribe Maleae conditioning resistance to P. expansum on genetic linkage group 3 (qM-Pe3.1) and linkage group 10 (qM-Pe10.1). These loci were identified in a M.× domestica 'Royal Gala' X M. sieversii PI613981 family (GMAL4593) based on blue mold lesion diameter seven days post-inoculation in mature, wounded apple fruit inoculated with P. expansum. Phenotypic analyses were conducted in 169 progeny over a four year period. PI613981 was the source of the resistance allele for qM-Pe3.1, a QTL with a major effect on blue mold resistance, accounting for 27.5% of the experimental variability. The QTL mapped from 67.3 to 74 cM on linkage group 3 of the GMAL4593 genetic linkage map. qM-Pe10.1 mapped from 73.6 to 81.8 cM on linkage group 10. It had less of an effect on resistance, accounting for 14% of the experimental variation. 'Royal Gala' was the primary contributor to the resistance effect of this QTL. However, resistance-associated alleles in both parents appeared to contribute to the least square mean blue mold lesion diameter in an additive manner at qM-Pe10.1. A GMAL4593 genetic linkage map composed of simple sequence repeats and 'Golden Delicious' single nucleotide polymorphism markers was able to detect qM-Pe10.1, but failed to detect qM-Pe3.1. The subsequent addition of genotyping-by-sequencing markers to the linkage map provided better coverage of the PI613981 genome on linkage group 3 and facilitated discovery of qM-Pe3.1. A DNA test for qM-Pe3.1 has been developed and is currently being evaluated for its ability to predict blue mold resistance in progeny segregating for qMPe3.1. Due to the long juvenility of apple, the availability of a DNA test to screen for the presence of qM-Pe3.1 at the seedling stage will greatly improve efficiency of breeding apple for blue mold resistance. © This is an open access article, free of all copyright, and may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. The work is made available under the Creative Commons CC0 public domain dedication.
Scientific Publication
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