נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Aconitase activity and expression during the development of lemon fruit
Year:
2000
Source of publication :
Physiologia Plantarum
Authors :
דהן, אסתר
;
.
צדקה, אבי
;
.
Volume :
108
Co-Authors:
Sadka, A., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Dahan, E., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Cohen, L., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Marsh, K.B., Horticultural and Food Research, Institute of New Zealand, Mt. Albert Research Center, 120 Mt. Albert Road, Auckland, New Zealand
Facilitators :
From page:
255
To page:
262
(
Total pages:
8
)
Abstract:
Citrus fruits are characterized by the accumulation of high levels of citric acid in the juice sac cells and a decline in acid level toward maturation. It has been suggested that changes in mitochondrial aconitase (EC 4.2.1.3) activity affect fruit acidity. Recently, a cytosolic aconitase (cyt-Aco) homologous to mammalian iron-regulated proteins was identified in plants, leading us to re-evaluate the role of aconitase in acid accumulation. Aconitase activity was studied in 2 contrasting citrus varieties, sweet lime (Citrus limettioides Tan., low acid) and sour lemon (Citrus limon var. Eureka, high acid). Two aconitase isozymes were detected. One declined early in sour lemon fruit development, but was constant throughout sweet lime fruit development. Its reduction in sour lemon was associated with a decrease in aconitase activity in the mitochondrial fraction. Another isozyme was detected in sour lemon toward maturation, and was associated with an increase in aconitase activity in the soluble fraction, suggesting a cytosolic localization. The cyt-Aco was cloned from lemon juice sac cells, but in contrast to the changes in isozyme activity, its expression was constant during fruit development. We present a model, which suggests that reduction of the mitochondrial aconitase activity plays a role in acid accumulation, while an increase in the cyt-Aco activity reduces acid level toward fruit maturation.
Note:
Related Files :
Citrus limon
Cytosol
enzyme activity
enzyme localization
isoenzyme
maturation
Model
ripening
עוד תגיות
תוכן קשור
More details
DOI :
10.1034/j.1399-3054.2000.108003255.x
Article number:
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
32810
Last updated date:
02/03/2022 17:27
Creation date:
17/04/2018 01:12
You may also be interested in
Scientific Publication
Aconitase activity and expression during the development of lemon fruit
108
Sadka, A., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Dahan, E., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Cohen, L., Department of Citriculture, Institute of Horticulture, Volcani Center, P.O. Box 6, Bet Dagan, 50250, Israel
Marsh, K.B., Horticultural and Food Research, Institute of New Zealand, Mt. Albert Research Center, 120 Mt. Albert Road, Auckland, New Zealand
Aconitase activity and expression during the development of lemon fruit
Citrus fruits are characterized by the accumulation of high levels of citric acid in the juice sac cells and a decline in acid level toward maturation. It has been suggested that changes in mitochondrial aconitase (EC 4.2.1.3) activity affect fruit acidity. Recently, a cytosolic aconitase (cyt-Aco) homologous to mammalian iron-regulated proteins was identified in plants, leading us to re-evaluate the role of aconitase in acid accumulation. Aconitase activity was studied in 2 contrasting citrus varieties, sweet lime (Citrus limettioides Tan., low acid) and sour lemon (Citrus limon var. Eureka, high acid). Two aconitase isozymes were detected. One declined early in sour lemon fruit development, but was constant throughout sweet lime fruit development. Its reduction in sour lemon was associated with a decrease in aconitase activity in the mitochondrial fraction. Another isozyme was detected in sour lemon toward maturation, and was associated with an increase in aconitase activity in the soluble fraction, suggesting a cytosolic localization. The cyt-Aco was cloned from lemon juice sac cells, but in contrast to the changes in isozyme activity, its expression was constant during fruit development. We present a model, which suggests that reduction of the mitochondrial aconitase activity plays a role in acid accumulation, while an increase in the cyt-Aco activity reduces acid level toward fruit maturation.
Scientific Publication
You may also be interested in