Co-Authors:
Rafaeli, A., Department of Entomology Faculty of Agriculture, The Hebrew University, Rehovot, 76-100, Israel
Soroker, V., Department of Entomology Faculty of Agriculture, The Hebrew University, Rehovot, 76-100, Israel
Abstract:
Heliothis armigera and Spodoptera littoralis females produced sex pheromone during their scotophase. Maximum levels of pheromone in Heliothis armigera were extracted from 2- to 3-day-old females during their 4.5-hr scotophase. On ligation between the head and the thorax of both species, normal pheromone production was inhibited. Homogenates of brains (consisting of brains, subesophageal ganglia, corpora cardiaca, and corpora allata) from both species induced a normal level of pheromone production by ligated females. Moreover, in S. littoralis, female brain hormone induced the production of the two components (Z)-9- and (Z, E)-9,11-tetradecenyl acetate tested by gas chromatography. Partial separation of brain homogenates, using low pressure C18-silica reversed-phase columns, produced a stable and active hormone source that gave a dose-dependent response. This hormone was found in brain extracts of both male and female H. armigera and S. littoralis females. In addition, S. littoralis induced pheromone production by H. armigera ligated females. © 1989 Plenum Publishing Corporation.