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פותח על ידי קלירמאש פתרונות בע"מ -
High Expression of CD200 and CD200R1 Distinguishes Stem and Progenitor Cell Populations within Mammary Repopulating Units
Year:
2018
Source of publication :
Stem Cell Reports
Authors :
ברש, איתמר
;
.
קודינוב, טניה
;
.
ראונר, גת
;
.
Volume :
11
Co-Authors:

Rauner, G., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel, Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY, United States; Kudinov, T., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel; Gilad, S., The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel; Hornung, G., The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

Facilitators :
From page:
288
To page:
302
(
Total pages:
15
)
Abstract:

Aiming to unravel the top of the mammary epithelial cell hierarchy, a subset of the CD49fhighCD24med mammary repopulating units (MRUs) was identified by flow cytometry, expressing high levels of CD200 and its receptor CD200R1. These MRUCD200/CD200R1 repopulated a larger area of de-epithelized mammary fat pads than the rest of the MRUs, termed MRUnot CD200/CD200R1. MRUCD200/CD200R1 maintained a much lower number of divergently defined, highly expressed genes and pathways that support better cell growth, development, differentiation, and progenitor activity than their MRUnot CD200/CD200R1 counterparts. A defined profile of hierarchically associated genes supporting a single-lineage hypothesis was confirmed by in vitro mammosphere analysis that assembled 114 genes with decreased expression from MRUCD200/CD200R1 via MRUnot CD200/CD200R1 toward CD200+CD200R1− and CD200R1+CD200− cells. About 40% of these genes were shared by a previously published database of upregulated genes in mammary/breast stem cells and may represent the core genes involved in mammary stemness. Barash and colleagues show that high expression levels of the immunoglobulin proteins CD200 and CD200R1 distinguish stem from progenitor cells with unique characteristics within the mammary repopulating units. This finding enables enrichment of the stem cell population. It contributes to elucidating the composition at the top of the mammary cell hierarchy that enables cyclic regeneration periods between lactations. © 2018 The Author(s)

Note:
Related Files :
CD200
CD200R1
Mammary gland
stem cells
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.stemcr.2018.05.013
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
34670
Last updated date:
02/03/2022 17:27
Creation date:
01/07/2018 11:54
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Scientific Publication
High Expression of CD200 and CD200R1 Distinguishes Stem and Progenitor Cell Populations within Mammary Repopulating Units
11

Rauner, G., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel, Baker Institute for Animal Health, Cornell University College of Veterinary Medicine, Ithaca, NY, United States; Kudinov, T., Institute of Animal Science, ARO, The Volcani Center, Bet-Dagan, Israel, The Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, Jerusalem, Israel; Gilad, S., The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel; Hornung, G., The Nancy & Stephen Grand Israel National Center for Personalized Medicine, Weizmann Institute of Science, Rehovot, Israel

High Expression of CD200 and CD200R1 Distinguishes Stem and Progenitor Cell Populations within Mammary Repopulating Units

Aiming to unravel the top of the mammary epithelial cell hierarchy, a subset of the CD49fhighCD24med mammary repopulating units (MRUs) was identified by flow cytometry, expressing high levels of CD200 and its receptor CD200R1. These MRUCD200/CD200R1 repopulated a larger area of de-epithelized mammary fat pads than the rest of the MRUs, termed MRUnot CD200/CD200R1. MRUCD200/CD200R1 maintained a much lower number of divergently defined, highly expressed genes and pathways that support better cell growth, development, differentiation, and progenitor activity than their MRUnot CD200/CD200R1 counterparts. A defined profile of hierarchically associated genes supporting a single-lineage hypothesis was confirmed by in vitro mammosphere analysis that assembled 114 genes with decreased expression from MRUCD200/CD200R1 via MRUnot CD200/CD200R1 toward CD200+CD200R1− and CD200R1+CD200− cells. About 40% of these genes were shared by a previously published database of upregulated genes in mammary/breast stem cells and may represent the core genes involved in mammary stemness. Barash and colleagues show that high expression levels of the immunoglobulin proteins CD200 and CD200R1 distinguish stem from progenitor cells with unique characteristics within the mammary repopulating units. This finding enables enrichment of the stem cell population. It contributes to elucidating the composition at the top of the mammary cell hierarchy that enables cyclic regeneration periods between lactations. © 2018 The Author(s)

Scientific Publication
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