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A local strain of Paprika mild mottle virus breaks L(3) resistance in peppers and is accelerated in Tomato brown rugose fruit virus-infected Tm-2(2)-resistant tomatoes
Year:
2018
Source of publication :
Virus Genes
Authors :
בקלמן, ילנה
;
.
דומברובסקי, אביב
;
.
לוריא, נטע
;
.
לכמן, עודד
;
.
סלע, נעה
;
.
סמית', אלישבע
;
.
Volume :
54
Co-Authors:

Koren, A

Facilitators :
From page:
280
To page:
289
(
Total pages:
10
)
Abstract:

During October 2014, unfamiliar mild mosaic and mottling symptoms were identified on leaves of pepper (Capsicum chinense cv. Habanero) seedlings grown in the Arava valley in Israel 2-3 weeks post planting. Symptomatic plants were tested positive by ELISA using laboratory-produced antisera for tobamovirus species. Typical tobamovirus rod-shaped morphology was observed by transmission electron microscopy (TEM) analysis of purified virion preparation that was used for mechanical inoculation of laboratory test plants for the completion of Koch's postulates. The complete viral genome was sequenced from small interfering RNA purified from symptomatic pepper leaves and fruits by next-generation sequencing (NGS) using Illumina MiSeq platform. The contigs generated by the assembly covered 80% of the viral genome. RT-PCR amplification and Sanger sequencing were employed in order to validate the sequence generated by NGS technology. The nucleotide sequence of the complete viral genome was 99% identical to the complete genome of Paprika mild mottle virus isolate from Japan (PaMMV-J), and the deduced amino acid sequence was 99% identical to PaMMV-J protein. Amplicons from seed RNA showed 100% identity to the viral isolate from the collected symptomatic pepper plants. Partial host range analysis revealed a slow development of systemic infection in inoculated tomato plants (Lycopersicon esculentum). Interestingly, double inoculation of susceptible wild-type tomato plants and Tm-22-resistant tomato plants with the PaMMV-IL and Tomato brown rugose fruit virus (ToBRFV) resulted in accelerated viral expression in the plants.

Note:
Related Files :
Capsicum annuum (paprika)
Solanum lycopersicum
Tobamovirus
virus transmission
עוד תגיות
תוכן קשור
More details
DOI :
 doi: 10.1007/s11262-018-1539-2
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
35775
Last updated date:
02/03/2022 17:27
Creation date:
29/07/2018 13:24
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Scientific Publication
A local strain of Paprika mild mottle virus breaks L(3) resistance in peppers and is accelerated in Tomato brown rugose fruit virus-infected Tm-2(2)-resistant tomatoes
54

Koren, A

A local strain of Paprika mild mottle virus breaks L(3) resistance in peppers and is accelerated in Tomato brown rugose fruit virus-infected Tm-2(2)-resistant tomatoes .

During October 2014, unfamiliar mild mosaic and mottling symptoms were identified on leaves of pepper (Capsicum chinense cv. Habanero) seedlings grown in the Arava valley in Israel 2-3 weeks post planting. Symptomatic plants were tested positive by ELISA using laboratory-produced antisera for tobamovirus species. Typical tobamovirus rod-shaped morphology was observed by transmission electron microscopy (TEM) analysis of purified virion preparation that was used for mechanical inoculation of laboratory test plants for the completion of Koch's postulates. The complete viral genome was sequenced from small interfering RNA purified from symptomatic pepper leaves and fruits by next-generation sequencing (NGS) using Illumina MiSeq platform. The contigs generated by the assembly covered 80% of the viral genome. RT-PCR amplification and Sanger sequencing were employed in order to validate the sequence generated by NGS technology. The nucleotide sequence of the complete viral genome was 99% identical to the complete genome of Paprika mild mottle virus isolate from Japan (PaMMV-J), and the deduced amino acid sequence was 99% identical to PaMMV-J protein. Amplicons from seed RNA showed 100% identity to the viral isolate from the collected symptomatic pepper plants. Partial host range analysis revealed a slow development of systemic infection in inoculated tomato plants (Lycopersicon esculentum). Interestingly, double inoculation of susceptible wild-type tomato plants and Tm-22-resistant tomato plants with the PaMMV-IL and Tomato brown rugose fruit virus (ToBRFV) resulted in accelerated viral expression in the plants.

Scientific Publication
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