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פותח על ידי קלירמאש פתרונות בע"מ -
Differential metabolism of L–phenylalanine in the formation of aromatic volatiles in melon (Cucumis melo L.) fruit
Year:
2018
Source of publication :
Phytochemistry
Authors :
בורגר, יוסף
;
.
בר, עינת
;
.
ג'יראד, פליאה
;
.
גונדה, איתי
;
.
דוידוביץ'-רקנאטי, רחל
;
.
ויסוצקי, גיא
;
.
לב, שרי
;
.
לוינסון, אפרים
;
.
משולם, יובל
;
.
פורטנוי, ויטלי
;
.
קציר, נורית
;
.
שפר, ארתור
;
.
תדמור, יעקב
;
.
Volume :
148
Co-Authors:

Giovannoni, J.J.; Fei, Z.; Fait, A.

Facilitators :
From page:
122
To page:
131
(
Total pages:
10
)
Abstract:

Studies on the active pathways and the genes involved in the biosynthesis of L-phenylalanine-derived volatiles in fleshy fruits are sparse. Melon fruit rinds converted stable-isotope labeled L-phe into more than 20 volatiles. Phenylpropanes, phenylpropenes and benzenoids are apparently produced via the well-known phenylpropanoid pathway involving phenylalanine ammonia lyase (PAL) and being (E)-cinnamic acid a key intermediate. Phenethyl derivatives seemed to be derived from L-phe via a separate biosynthetic route not involving (E)-cinnamic acid and PAL. To explore for a biosynthetic route to (E)-cinnamaldehyde in melon rinds, soluble protein cell-free extracts were assayed with (E)-cinnamic acid, CoA, ATP, NADPH and MgSO4, producing (E)-cinnamaldehyde in vitro. In this context, we characterized CmCNL, a gene encoding for (E)-cinnamic acid:coenzyme A ligase, inferred to be involved in the biosynthesis of (E)-cinnamaldehyde. Additionally we describe CmBAMT, a SABATH gene family member encoding a benzoic acid:S-adenosyl-L-methionine carboxyl methyltransferase having a role in the accumulation of methyl benzoate. Our approach leads to a more comprehensive understanding of L-phe metabolism into aromatic volatiles in melon fruit. © 2018 Elsevier Ltd

Note:
Related Files :
Cucumis melo L.
Cucurbitaceae
Melon
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1016/j.phytochem.2017.12.018
Article number:
0
Affiliations:

Institute of Plant Sciences, Newe Ya'ar Research Center, Agricultural Research Organization, The Volcani Center, P.O. Box 1021, Ramat Yishay, Israel; The Jacob Blaustein Institutes for Desert Research, Ben-Gurion University of the Negev, Beer-Sheva, Israel; ORT Braude College, Department of Biotechnology Engineering, Karmiel, Israel; Institute of Plant Sciences, The Volcani Center, Agricultural Research Organization, P.O. Box 6, Rishon LeZion, Israel; Boyce Thompson Institute, Cornell University, Ithaca, NY, United States; USDA-ARS Robert W. Holley Center for Agriculture and Health, Ithaca, NY, United States

Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
36349
Last updated date:
02/03/2022 17:27
Creation date:
05/08/2018 09:39
Scientific Publication
Differential metabolism of L–phenylalanine in the formation of aromatic volatiles in melon (Cucumis melo L.) fruit
148

Giovannoni, J.J.; Fei, Z.; Fait, A.

Differential metabolism of L–phenylalanine in the formation of aromatic volatiles in melon (Cucumis melo L.) fruit

Studies on the active pathways and the genes involved in the biosynthesis of L-phenylalanine-derived volatiles in fleshy fruits are sparse. Melon fruit rinds converted stable-isotope labeled L-phe into more than 20 volatiles. Phenylpropanes, phenylpropenes and benzenoids are apparently produced via the well-known phenylpropanoid pathway involving phenylalanine ammonia lyase (PAL) and being (E)-cinnamic acid a key intermediate. Phenethyl derivatives seemed to be derived from L-phe via a separate biosynthetic route not involving (E)-cinnamic acid and PAL. To explore for a biosynthetic route to (E)-cinnamaldehyde in melon rinds, soluble protein cell-free extracts were assayed with (E)-cinnamic acid, CoA, ATP, NADPH and MgSO4, producing (E)-cinnamaldehyde in vitro. In this context, we characterized CmCNL, a gene encoding for (E)-cinnamic acid:coenzyme A ligase, inferred to be involved in the biosynthesis of (E)-cinnamaldehyde. Additionally we describe CmBAMT, a SABATH gene family member encoding a benzoic acid:S-adenosyl-L-methionine carboxyl methyltransferase having a role in the accumulation of methyl benzoate. Our approach leads to a more comprehensive understanding of L-phe metabolism into aromatic volatiles in melon fruit. © 2018 Elsevier Ltd

Scientific Publication
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