תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםThe chloroform fumigation-extraction method for determining soil microbial biomass is commonly applied at soil moisture (SM) levels of 40–50% water-holding capacity (WHC). Below that moisture range, restricted enzymatic autolysis might limit the fumigation flush of extractable cellular material, thus underestimating microbial biomass. Likewise, the upper range of SM (i.e., >50% WHC) is considered problematic due to an alleged reduced fumigation efficiency when vapor diffusivity through soil is reduced. The current investigation demonstrated relatively constant fumigation flushes along a 40–90% WHC moisture gradient alongside unchanging carbon-to-nitrogen flush ratios corresponding to characteristic cellular values. This clearly refuted the proposed reduced fumigation efficiency under elevated moisture conditions, and thus validated the applicability of the method to samples collected moist. © 2018 Elsevier B.V.
Department of Soil Chemistry, Plant Nutrition and Microbiology, Institute of Soil, Water and Environmental Sciences, Volcani Center, Agricultural Research Organization (ARO), Rishon LeZion, Israel; Department of Soil and Water Sciences, Faculty of Agriculture, Environment and Food Sciences, The Hebrew University of Jerusalem, Rehovot, Israel
The chloroform fumigation-extraction method for determining soil microbial biomass is commonly applied at soil moisture (SM) levels of 40–50% water-holding capacity (WHC). Below that moisture range, restricted enzymatic autolysis might limit the fumigation flush of extractable cellular material, thus underestimating microbial biomass. Likewise, the upper range of SM (i.e., >50% WHC) is considered problematic due to an alleged reduced fumigation efficiency when vapor diffusivity through soil is reduced. The current investigation demonstrated relatively constant fumigation flushes along a 40–90% WHC moisture gradient alongside unchanging carbon-to-nitrogen flush ratios corresponding to characteristic cellular values. This clearly refuted the proposed reduced fumigation efficiency under elevated moisture conditions, and thus validated the applicability of the method to samples collected moist. © 2018 Elsevier B.V.
