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פותח על ידי קלירמאש פתרונות בע"מ -
Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium
Year:
2018
Authors :
אלבז, מיכל
;
.
גרשון, ערן
;
.
Volume :
16
Co-Authors:

Elbaz, M., Department of Ruminant Science, Agricultural Research Organization, Rishon LeZion, Israel; Hadas, R., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel; Bilezikjian, L.M., Clayton Foundation Laboratories for Peptide Biology, Laboratory of Neuronal Structure and Function, Salk Institute for Biological Studies, La Jolla, San Diego, CA, United States; Gershon, E., Department of Ruminant Science, Agricultural Research Organization, Rishon LeZion, Israel

Facilitators :
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Total pages:
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Abstract:

Background: Forkhead Transcription Factor L2 (FOXL2) is a member of the forkhead family with important roles in reproduction. Recent studies showed that FOXL2 is expressed in human and bovine endometrium and that its levels fluctuate during pregnancy. In this study, we aimed at evaluating the expression and function of FOXL2 in embryo implantation. Methods: Mouse uteri at different days of pregnancy were isolated and analyzed for the expression and localization of FOXL2. A lentiviral strategy was further employed to either knockdown or overexpress FOXL2 in non-receptive human endometrial AN3-CA cells and in receptive Ishikawa cells, respectively. These genetically modified cells were compared to cells infected with a control lentivirus to determine the function of FOXL2 in trophectoderm cells adherence to Endometrial Epithelium was associated with the expression of genes known to be involved in acquisition of uterine receptivity. Results: We report that FOXL2 is expressed in both, the luminal epithelium and the myometrium of the mouse uterus and that its expression declines prior to implantation. We found that endometrial cells expressing low FOXL2 levels, either endogenous or genetically manipulated, were associated with a higher attachment rate of mouse blastocysts or human Jeg3 spheroids and mouse blastocysts. In accordance, low-FOXL2 levels were associated with changes in the expression level of components of the Wnt/Fzd and apoptotic pathways, both of which are involved in uterine receptivity. Furthermore, FOXL2 expression was inversely correlated with G-protein signaling protein 2 (Rgs2) and cytokine expression. Conclusions: These results suggest that FOXL2 interferes with embryo attachment. Better understanding of the function of FOXL2 in the uterus would possibly suggest novel strategies for treatment of infertility attributed to repeated implantation failure. © 2018 The Author(s).

Note:
Related Files :
Cell adherence
Embryo attachment
FOXL2
FOXL2 depletion
FOXL2 overexpression
gene expression
Uterine receptivity
עוד תגיות
תוכן קשור
More details
DOI :
10.1186/s12958-018-0329-y
Article number:
12
Affiliations:

Department of Ruminant Science, Agricultural Research Organization, Rishon LeZion, Israel; Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel; Clayton Foundation Laboratories for Peptide Biology, Laboratory of Neuronal Structure and Function, Salk Institute for Biological Studies, La Jolla, San Diego, CA, United States

Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
36683
Last updated date:
02/03/2022 17:27
Creation date:
16/08/2018 10:56
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Scientific Publication
Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium
16

Elbaz, M., Department of Ruminant Science, Agricultural Research Organization, Rishon LeZion, Israel; Hadas, R., Department of Biological Regulation, Weizmann Institute of Science, Rehovot, Israel; Bilezikjian, L.M., Clayton Foundation Laboratories for Peptide Biology, Laboratory of Neuronal Structure and Function, Salk Institute for Biological Studies, La Jolla, San Diego, CA, United States; Gershon, E., Department of Ruminant Science, Agricultural Research Organization, Rishon LeZion, Israel

Uterine Foxl2 regulates the adherence of the Trophectoderm cells to the endometrial epithelium

Background: Forkhead Transcription Factor L2 (FOXL2) is a member of the forkhead family with important roles in reproduction. Recent studies showed that FOXL2 is expressed in human and bovine endometrium and that its levels fluctuate during pregnancy. In this study, we aimed at evaluating the expression and function of FOXL2 in embryo implantation. Methods: Mouse uteri at different days of pregnancy were isolated and analyzed for the expression and localization of FOXL2. A lentiviral strategy was further employed to either knockdown or overexpress FOXL2 in non-receptive human endometrial AN3-CA cells and in receptive Ishikawa cells, respectively. These genetically modified cells were compared to cells infected with a control lentivirus to determine the function of FOXL2 in trophectoderm cells adherence to Endometrial Epithelium was associated with the expression of genes known to be involved in acquisition of uterine receptivity. Results: We report that FOXL2 is expressed in both, the luminal epithelium and the myometrium of the mouse uterus and that its expression declines prior to implantation. We found that endometrial cells expressing low FOXL2 levels, either endogenous or genetically manipulated, were associated with a higher attachment rate of mouse blastocysts or human Jeg3 spheroids and mouse blastocysts. In accordance, low-FOXL2 levels were associated with changes in the expression level of components of the Wnt/Fzd and apoptotic pathways, both of which are involved in uterine receptivity. Furthermore, FOXL2 expression was inversely correlated with G-protein signaling protein 2 (Rgs2) and cytokine expression. Conclusions: These results suggest that FOXL2 interferes with embryo attachment. Better understanding of the function of FOXL2 in the uterus would possibly suggest novel strategies for treatment of infertility attributed to repeated implantation failure. © 2018 The Author(s).

Scientific Publication