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Dennis J Gray, Michael E Compton, Ernest Hiebert, Chia-Min Lin, Victor P Gaba

As described in Chapter 19, several techniques have been utilized successfully to introduce
DNA into plant cells. Of these methods, particle bombardment, wherein microscopic metal
particles coated with genetically engineered DNA are explosively accelerated into plant
cells, has become the second most widely used vehicle for plant genetic transformation,
after Agrobacterium-mediated transformation (Gray and Finer, 1993). Several distinct
“particle guns” have been described, including the Biolistic PDS 1000/He (Kikkert, 1993),
which is the only commercially available device. The most attractive of the noncommercial
devices is the particle inflow gun (PIG)(Finer et al., 1992), based on a flowing helium device
described by Takeuchi et al.(1992), which can be fabricated from steel plate with readily
available parts and offers performance on par with the Biolistic PDS 1000/He (Brown et al...

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Construction and Use of a Simple Gene Gun for Particle Bombardment (chapter 21)

Dennis J Gray, Michael E Compton, Ernest Hiebert, Chia-Min Lin, Victor P Gaba

Construction and Use of a Simple Gene Gun for Particle Bombardment (chapter 21)

As described in Chapter 19, several techniques have been utilized successfully to introduce
DNA into plant cells. Of these methods, particle bombardment, wherein microscopic metal
particles coated with genetically engineered DNA are explosively accelerated into plant
cells, has become the second most widely used vehicle for plant genetic transformation,
after Agrobacterium-mediated transformation (Gray and Finer, 1993). Several distinct
“particle guns” have been described, including the Biolistic PDS 1000/He (Kikkert, 1993),
which is the only commercially available device. The most attractive of the noncommercial
devices is the particle inflow gun (PIG)(Finer et al., 1992), based on a flowing helium device
described by Takeuchi et al.(1992), which can be fabricated from steel plate with readily
available parts and offers performance on par with the Biolistic PDS 1000/He (Brown et al...

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