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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
An In Vitro Selection Method for a Melon Variety which Regenerates by Direct Organogenesis
Year:
1992
Source of publication :
Cucurbit Genetics Cooperative
Authors :
אנטיגנוס, יחזקאל
;
.
גאבה, ויקטור
;
.
Volume :
15
Co-Authors:
Facilitators :
From page:
65
To page:
66
(
Total pages:
2
)
Abstract:

Materials and methods. Seeds of Cucumis melo L. cv Galia were pealed and surface
sterilised in 1.2% solution of hypochlorite, 1 drop of Tween 20 per 100 ml. Seeds were
washed 4X with sterile water and cut into 4 longitudinally through the embryo, each explant
thus being half of a cotyledon with attached embryo fragment. The primary explants were
then plated onto autoclaved Murashige and Skoog (8) medium, with 3% sucrose, 8-10 g/l
agar, and 1 mg/1 benzyl adenine (MSBA1 medium). Plant material was incubated in a
growth room at 26 C in continuous light. Explants were transferred to fresh MSBA1 medium
with kanamycin at periods up to 7d after primary explant preparation. In a second set of
experiments explants were cut in pieces prior to transfer to kanamycin-containing MSBA1 at
5d old (table 2). Regeneration was scored as the% of explants with shoots or shoot buds...

Note:
Related Files :
aminoglycoside antibiotics
Antibiotics
biotechnology
genetic engineering
kanamycin
Melons
tissue culture
vegetables
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
36703
Last updated date:
02/03/2022 17:27
Creation date:
19/08/2018 12:27
Scientific Publication
An In Vitro Selection Method for a Melon Variety which Regenerates by Direct Organogenesis
15
An In Vitro Selection Method for a Melon Variety which Regenerates by Direct Organogenesis

Materials and methods. Seeds of Cucumis melo L. cv Galia were pealed and surface
sterilised in 1.2% solution of hypochlorite, 1 drop of Tween 20 per 100 ml. Seeds were
washed 4X with sterile water and cut into 4 longitudinally through the embryo, each explant
thus being half of a cotyledon with attached embryo fragment. The primary explants were
then plated onto autoclaved Murashige and Skoog (8) medium, with 3% sucrose, 8-10 g/l
agar, and 1 mg/1 benzyl adenine (MSBA1 medium). Plant material was incubated in a
growth room at 26 C in continuous light. Explants were transferred to fresh MSBA1 medium
with kanamycin at periods up to 7d after primary explant preparation. In a second set of
experiments explants were cut in pieces prior to transfer to kanamycin-containing MSBA1 at
5d old (table 2). Regeneration was scored as the% of explants with shoots or shoot buds...

Scientific Publication
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