תפריט נגישות
ניגודיות עדינהניגודיות גבוהה
מונוכרום
הדגשת קישורים
חסימת אנימציה
פונט קריא
סגור
איפוס הגדרות נגישות
להורדת מודול נגישות חינם תפריט נגישותניגודיות עדינהניגודיות גבוההמונוכרוםהדגשת קישוריםחסימת אנימציהפונט קריאסגוראיפוס הגדרות נגישותלהורדת מודול נגישות חינםMaterials and methods. Seeds of Cucumis melo L. cv Galia were pealed and surface
sterilised in 1.2% solution of hypochlorite, 1 drop of Tween 20 per 100 ml. Seeds were
washed 4X with sterile water and cut into 4 longitudinally through the embryo, each explant
thus being half of a cotyledon with attached embryo fragment. The primary explants were
then plated onto autoclaved Murashige and Skoog (8) medium, with 3% sucrose, 8-10 g/l
agar, and 1 mg/1 benzyl adenine (MSBA1 medium). Plant material was incubated in a
growth room at 26 C in continuous light. Explants were transferred to fresh MSBA1 medium
with kanamycin at periods up to 7d after primary explant preparation. In a second set of
experiments explants were cut in pieces prior to transfer to kanamycin-containing MSBA1 at
5d old (table 2). Regeneration was scored as the% of explants with shoots or shoot buds...
Materials and methods. Seeds of Cucumis melo L. cv Galia were pealed and surface
sterilised in 1.2% solution of hypochlorite, 1 drop of Tween 20 per 100 ml. Seeds were
washed 4X with sterile water and cut into 4 longitudinally through the embryo, each explant
thus being half of a cotyledon with attached embryo fragment. The primary explants were
then plated onto autoclaved Murashige and Skoog (8) medium, with 3% sucrose, 8-10 g/l
agar, and 1 mg/1 benzyl adenine (MSBA1 medium). Plant material was incubated in a
growth room at 26 C in continuous light. Explants were transferred to fresh MSBA1 medium
with kanamycin at periods up to 7d after primary explant preparation. In a second set of
experiments explants were cut in pieces prior to transfer to kanamycin-containing MSBA1 at
5d old (table 2). Regeneration was scored as the% of explants with shoots or shoot buds...
