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פותח על ידי קלירמאש פתרונות בע"מ -
Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit
Year:
2018
Source of publication :
Plant Journal
Authors :
בלאוסוב, אדוארד
;
.
דורון-פייגנבאום, עדי
;
.
יסלזון, ילנה
;
.
כהן, דביר
;
.
כהן, שחר
;
.
לוין, אילן
;
.
מוי, מיכל
;
.
פטרייקוב, מרינה
;
.
שמאי, אריק
;
.
שפר, ארתור
;
.
Volume :
96
Co-Authors:

Efrati, A., Zeraim-Syngenta Seed Co., Gedera, Israel; Houminer, N., Zeraim-Syngenta Seed Co., Gedera, Israel; Bar, M., Zeraim-Syngenta Seed Co., Gedera, Israel; Ast, T., Department of Molecular Genetics, Weizmann Institute, Rehovot, Israel; Schuldiner, M., Department of Molecular Genetics, Weizmann Institute, Rehovot, Israel; Klemens, P.A.W., Department of Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany; Neuhaus, E., Department of Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany; Baxter, C.J., Syngenta Seed Co., Jeallott's Hill Research Centre, Bracknell, United Kingdom; Rickett, D., Syngenta Seed Co., Jeallott's Hill Research Centre, Bracknell, United Kingdom; Bonnet, J., Syngenta Seed Co., Toulouse Innovation Center, Saint Sauveur, France; White, R., USDA-ARS and 

Facilitators :
From page:
343
To page:
357
(
Total pages:
15
)
Abstract:

The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality. Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose. As fructose is perceived as approximately twice as sweet as glucose, increasing its concentration at the expense of glucose can improve tomato fruit taste. Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels. In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines. The results indicated that a SWEET protein was strongly upregulated in the lines with a high fructose-to-glucose ratio. Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein. The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose. These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels. This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit. The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms. © 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd

Note:
Related Files :
Cell membranes
fructose
Fruits
gene expression
proteins
Solanum lycopersicum
tomato
Transgenic tomato plants
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/tpj.14035
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
37378
Last updated date:
02/03/2022 17:27
Creation date:
02/10/2018 10:58
Scientific Publication
Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit
96

Efrati, A., Zeraim-Syngenta Seed Co., Gedera, Israel; Houminer, N., Zeraim-Syngenta Seed Co., Gedera, Israel; Bar, M., Zeraim-Syngenta Seed Co., Gedera, Israel; Ast, T., Department of Molecular Genetics, Weizmann Institute, Rehovot, Israel; Schuldiner, M., Department of Molecular Genetics, Weizmann Institute, Rehovot, Israel; Klemens, P.A.W., Department of Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany; Neuhaus, E., Department of Plant Physiology, University of Kaiserslautern, Kaiserslautern, Germany; Baxter, C.J., Syngenta Seed Co., Jeallott's Hill Research Centre, Bracknell, United Kingdom; Rickett, D., Syngenta Seed Co., Jeallott's Hill Research Centre, Bracknell, United Kingdom; Bonnet, J., Syngenta Seed Co., Toulouse Innovation Center, Saint Sauveur, France; White, R., USDA-ARS and 

Natural genetic variation for expression of a SWEET transporter among wild species of Solanum lycopersicum (tomato) determines the hexose composition of ripening tomato fruit

The sugar content of Solanum lycopersicum (tomato) fruit is a primary determinant of taste and quality. Cultivated tomato fruit are characterized by near-equimolar levels of the hexoses glucose and fructose, derived from the hydrolysis of translocated sucrose. As fructose is perceived as approximately twice as sweet as glucose, increasing its concentration at the expense of glucose can improve tomato fruit taste. Introgressions of the FgrH allele from the wild species Solanum habrochaites (LA1777) into cultivated tomato increased the fructose-to-glucose ratio of the ripe fruit by reducing glucose levels and concomitantly increasing fructose levels. In order to identify the function of the Fgr gene, we combined a fine-mapping strategy with RNAseq differential expression analysis of near-isogenic tomato lines. The results indicated that a SWEET protein was strongly upregulated in the lines with a high fructose-to-glucose ratio. Overexpressing the SWEET protein in transgenic tomato plants dramatically reduced the glucose levels and increased the fructose : glucose ratio in the developing fruit, thereby proving the function of the protein. The SWEET protein was localized to the plasma membrane and expression of the SlFgr gene in a yeast line lacking native hexose transporters complemented growth with glucose, but not with fructose. These results indicate that the SlFgr gene encodes a plasma membrane-localized glucose efflux transporter of the SWEET family, the overexpression of which reduces glucose levels and may allow for increased fructose levels. This article identifies the function of the tomato Fgr gene as a SWEET transporter, the upregulation of which leads to a modified sugar accumulation pattern in the fleshy fruit. The results point to the potential of the inedible wild species to improve fruit sugar accumulation via sugar transport mechanisms. © 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd

Scientific Publication
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