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פותח על ידי קלירמאש פתרונות בע"מ -
Parasitism of Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii-scanning electron microscopy and fluorescence microscopy
Year:
1983
Source of publication :
Phytopathology
Authors :
אלעד, יגאל
;
.
Volume :
73
Co-Authors:

I Chet, P Boyle, Y Henis

Facilitators :
From page:
85
To page:
88
(
Total pages:
4
)
Abstract:

Hyphal interactions between either Trichoderma harzianum or T. hamatum, and Sclerotium rolfsii or Rhizoctonia solani were observed by scanning electron microscopy. Trichoderma spp. attached to the host either by hyphal coils, hooks, or appressoria. Lysed sites and penetration holes were found in hyphae of the plant pathogenic fungi, following removal of parasitic hyphae. High β-(1,3) glucanase and chitinase activities were detected in dual agar cultures when T. harzianum parasitized S. rolfsii, compared with low levels found with either fungus alone. In the presence of cycloheximide, antagonism was prevented and enzymatic activity was diminished. Interaction sites were stained by fluorescein isothiocyanate-conjugated lectins or Calcofluor White M2R New. Appearance of fluorescence indicated the presence of localized cell wall lysis at points of interaction between the antagonist and its host.

Note:
Related Files :
electron microscopy
Parasitism
Rhizoctonia
Rhizoctonia solani
Sclerotium rolfsii
soilborne plant pathogens
Trichoderma
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/Phyto-73-85
Article number:
0
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
37638
Last updated date:
02/03/2022 17:27
Creation date:
23/10/2018 13:40
Scientific Publication
Parasitism of Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii-scanning electron microscopy and fluorescence microscopy
73

I Chet, P Boyle, Y Henis

Parasitism of Trichoderma spp. on Rhizoctonia solani and Sclerotium rolfsii-scanning electron microscopy and fluorescence microscopy

Hyphal interactions between either Trichoderma harzianum or T. hamatum, and Sclerotium rolfsii or Rhizoctonia solani were observed by scanning electron microscopy. Trichoderma spp. attached to the host either by hyphal coils, hooks, or appressoria. Lysed sites and penetration holes were found in hyphae of the plant pathogenic fungi, following removal of parasitic hyphae. High β-(1,3) glucanase and chitinase activities were detected in dual agar cultures when T. harzianum parasitized S. rolfsii, compared with low levels found with either fungus alone. In the presence of cycloheximide, antagonism was prevented and enzymatic activity was diminished. Interaction sites were stained by fluorescein isothiocyanate-conjugated lectins or Calcofluor White M2R New. Appearance of fluorescence indicated the presence of localized cell wall lysis at points of interaction between the antagonist and its host.

Scientific Publication
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