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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Trichoderma and soil solarization induced microbial changes on plant surfaces
Year:
2006
Source of publication :
IOBC/WPRS Bulletin
Authors :
אוקון לוי, נטע
;
.
אלעד, יגאל
;
.
Volume :
29 (2)
Co-Authors:

 J Katan, SC Baker, JL Faull

Facilitators :
From page:
21
To page:
26
(
Total pages:
6
)
Abstract:

Both soil solarization and Trichoderma reduce disease incidence. In one work we studied
the effect of both treatments on rhizosphere populations and in a second work we studied the effect of
Trichoderma on phyllosphere populations. In both works the effect of the treatments on foliar
pathogens was studied. When the roots were in contact with the solarized soil or Trichoderma and the
foliage was inoculated with pathogens, strawberry, cucumber and common bean grown on treated
media showed significant reduction in disease after leaf inoculation with Botrytis cinerea or with
Sphaerotheca fuliginea, hence indicating induced resistance. Attempts were made to find the
relationship between indigenous populations of microorganisms in the rhizosphere or in the
phyllosphere and disease control. For this purpose we used a molecular approach based on 16S-rDNA
and denaturizing gradient gel electrophoresis (DGGE). Bacterial 16S ribosomal DNA (rDNA)
extraction from roots and leaves that were subjected to the various treatments was PCR amplified with
chosen primers. Amplicons were separated by size and base composition by DGGE in order to
fingerprint shifts in the structure of the natural plant-associated microbial communities that may result
from the treatments. The soil treatments resulted in changes in the DGGE patterns of rhizosphere and
phyllosphere populations. Some bacilli, pseudomonads and actinobacteria were detected and their role
in induced resistance is currently tested. T. harzianum treatment to leaves or roots resulted in increased
variability in the bacterial population inhabiting the leaves. It is suggested that some of the effect
exerted by soil solarization and Trichoderma are associated with microbial changes.

Note:
Related Files :
foliar diseases
Induced resistance
Phyllosphere
rhizosphere
Trichoderma
Trichoderma harzianum
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
37689
Last updated date:
02/03/2022 17:27
Creation date:
25/10/2018 09:39
Scientific Publication
Trichoderma and soil solarization induced microbial changes on plant surfaces
29 (2)

 J Katan, SC Baker, JL Faull

Trichoderma and soil solarization induced microbial changes on plant surfaces

Both soil solarization and Trichoderma reduce disease incidence. In one work we studied
the effect of both treatments on rhizosphere populations and in a second work we studied the effect of
Trichoderma on phyllosphere populations. In both works the effect of the treatments on foliar
pathogens was studied. When the roots were in contact with the solarized soil or Trichoderma and the
foliage was inoculated with pathogens, strawberry, cucumber and common bean grown on treated
media showed significant reduction in disease after leaf inoculation with Botrytis cinerea or with
Sphaerotheca fuliginea, hence indicating induced resistance. Attempts were made to find the
relationship between indigenous populations of microorganisms in the rhizosphere or in the
phyllosphere and disease control. For this purpose we used a molecular approach based on 16S-rDNA
and denaturizing gradient gel electrophoresis (DGGE). Bacterial 16S ribosomal DNA (rDNA)
extraction from roots and leaves that were subjected to the various treatments was PCR amplified with
chosen primers. Amplicons were separated by size and base composition by DGGE in order to
fingerprint shifts in the structure of the natural plant-associated microbial communities that may result
from the treatments. The soil treatments resulted in changes in the DGGE patterns of rhizosphere and
phyllosphere populations. Some bacilli, pseudomonads and actinobacteria were detected and their role
in induced resistance is currently tested. T. harzianum treatment to leaves or roots resulted in increased
variability in the bacterial population inhabiting the leaves. It is suggested that some of the effect
exerted by soil solarization and Trichoderma are associated with microbial changes.

Scientific Publication
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