Trichoderma isolates are known for their ability to control plant pathogens. In a previous study, we showed that various isolates of Trichoderma, including isolate T39 from the commercial biological control product TRICHODEX, were effective in controlling anthracnose (Colletotrichum acutatum) and grey mould (Botrytis cinerea) in strawberry under laboratory and greenhouse conditions. Three selected Trichoderma strains (T-39, T-161 and T-166) were assessed in a semi-commercial field experiment in Israel using different timing and dosage rates for suppression of strawberry anthracnose. All possible combinations of single, double or triple mixtures of the Trichoderma strains, applied at 0.4 and 0.8% concentrations and at 7- or 10-day intervals, were tested. Anthracnose severity (i.e., the number of diseased plants, apexes and petioles) was reduced by all possible combinations of the applied Trichoderma strains compared with the untreated controls. A similar field experiment was conducted to evaluate the efficacy of these strains for the control of grey mould. The reduction of grey mould was highest when the Trichodermastrains were applied at 2-day intervals at 0.4% concentration. In experiments attempting to determine the survival dynamics of populations of the Trichodermaisolates applied in mixtures, PCR using repeat motif primers was conducted. In these experiments, strawberry leaves were treated with a mixture of the isolates and dilution plating was done on plates containing a Trichoderma-selective medium. The strains were identified according to PCR band patterns typical for each Trichoderma isolate and compared with applications of sole isolates. A molecular approach, using PCR methodology, has been initiated for species identification of selected and reference Trichoderma strains.