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Gur Amit and Dani Zamir

The genetic variation underlying quantitative traits results from the segregation of numerous interacting quantitative trait loci (QTLs) whose expression is modified by the environment. Using a population derived from the green fruited tomato species L. pennellii, we identified 23 QTLs originating in the wild species, that are responsible for an increase in the total soluble solids (brix) of the fruit. To uncover the molecular basis of this variation, we characterised one of the QTLs, Brix9-2-5. The wild species allele increased glucose and fructose contents in cultivated tomato fruits in various genetic backgrounds and environments. Using nearly isogenic lines (NILs) for the QTL, high-resolution mapping analysis delimited Brix9-2-5 to a SNP (single nucleotide polymorphism)- defined recombination hotspot of 484 bp spanning an exon and intron of a fruit-specific apoplastic invertase. These results demonstrate a link between naturally occurring DNA. variation and a Mendelian determinant of a complex phenotype for a yield-associated trait.

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PHENOTYPING, FINE MAPPING AND CLONING OF A WILD SPECIES QTL THAT INCREASES HORTICULTURAL YIELD IN TOMATOES
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Gur Amit and Dani Zamir

PHENOTYPING, FINE MAPPING AND CLONING OF A WILD SPECIES QTL THAT INCREASES HORTICULTURAL YIELD IN TOMATOES

The genetic variation underlying quantitative traits results from the segregation of numerous interacting quantitative trait loci (QTLs) whose expression is modified by the environment. Using a population derived from the green fruited tomato species L. pennellii, we identified 23 QTLs originating in the wild species, that are responsible for an increase in the total soluble solids (brix) of the fruit. To uncover the molecular basis of this variation, we characterised one of the QTLs, Brix9-2-5. The wild species allele increased glucose and fructose contents in cultivated tomato fruits in various genetic backgrounds and environments. Using nearly isogenic lines (NILs) for the QTL, high-resolution mapping analysis delimited Brix9-2-5 to a SNP (single nucleotide polymorphism)- defined recombination hotspot of 484 bp spanning an exon and intron of a fruit-specific apoplastic invertase. These results demonstrate a link between naturally occurring DNA. variation and a Mendelian determinant of a complex phenotype for a yield-associated trait.

Scientific Publication
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