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פותח על ידי קלירמאש פתרונות בע"מ -
Successful floral-dipping transformation of post-anthesis lisianthus (Eustoma grandiflorum) flowers
Year:
2018
Source of publication :
Plant Journal
Authors :
אהי-ארומוסלה, סוניה
;
.
אוליבה, מורן
;
.
אורן-שמיר, מיכל
;
.
ארזי, צחי
;
.
פאנג, פאנג
;
.
Volume :
96
Co-Authors:

Zaccai, M., Department of Life Sciences, Ben Gurion University of the Negev, PO Box 653, Beersheva, Israel

Facilitators :
From page:
869
To page:
879
(
Total pages:
11
)
Abstract:

The adaptation of the Agrobacterium-mediated floral-dipping technique is limited, to date, to a small number of plants. In this paper, we present the efficient transformation of one of the leading plants in the cut flower industry, lisianthus (Eustoma grandiflorum). This method is approximately 18 months shorter than the known tissue culture-based transformation. The Excalibur Pink cultivar and two additional breeding lines, X-1042 and X-2541, were transformed using three different marker genes (benzyl alcohol acetyltransferase (BEAT) originating from Clarkia breweri, the feedback-insensitive bacterial gene AroG*, and the empty pART27 vector expressing a kanamycin-resistance cassette (nptII)). Genomic transformation was successful in all tested cases with transformation efficiency ranked from 0.2 to 2.9%, which is well in the range of results from Arabidopsis studies. Unlike Arabidopsis, in which floral-dipping transformation was efficient only at a pre-anthesis stage before ovary sealing, lisianthus flowers were transformed when dipping occurred 4 days pre-anthesis or 3−5 days post-anthesis with 1.5 and 3.7% efficiencies, respectively. Post-anthesis transformation occurred when the flower ovaries were sealed. Flower dipping of Excalibur Pink flowers with fluorescent Agrobacterium containing a GFP marker gene demonstrated Agrobacterium entrance into the sealed flower ovary through the open stigma and style tube. In this study, we demonstrated floral-dipping transformation of a commercial plant, lisianthus Excalibur Pink, occurring after sealing of the ovaries, probably via the stigma and wide open style tunnel. © 2018 The Authors The Plant Journal © 2018 John Wiley & Sons Ltd

Note:
Related Files :
Efficiency
Eustoma grandiflorum
Excalibur Pink
Genes
Plants (botany)
tissue culture
Transformation efficiency
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/tpj.14076
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
37737
Last updated date:
02/03/2022 17:27
Creation date:
29/10/2018 10:32
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