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To B or not to B: Comparative genomics suggests Arsenophonusas a source of B vitamins in whiteflies
Year:
2018
Source of publication :
Frontiers in Microbiology
Authors :
פרייליך, שירי
;
.
צחורי-פיין, עינת
;
.
Volume :
Co-Authors:

Santos-Garcia, D., Department of Entomology, Hebrew University of Jerusalem, Rehovot, Israel; Juravel, K., Department of Entomology, Hebrew University of Jerusalem, Rehovot, Israel;  Latorre, A., Institute for Integrative Systems Biology, Universitat de València-CSIC, València, Spain, Unidad Mixta de Investigación en Genómica y Salud, Fundación para El Fomento de la Investigación Sanit. y Biomédica de la Comunidad Valenciana (FISABIO), Institute for Integrative Systems Biology, Universitat de València, València, Spain; Moya, A., Institute for Integrative Systems Biology, Universitat de València-CSIC, València, Spain, Unidad Mixta de Investigación en Genómica y Salud, Fundación para El Fomento de la Investigación Sanit. y Biomédica de la Comunidad Valenciana (FISABIO), Institute for Integrative Systems Biology, Universitat de València, València, Spain; Morin, S., Department of Entomology, Hebrew University of Jerusalem, Rehovot, Israel; Silva, F.J., Institute for Integrative Systems Biology, Universitat de València-CSIC, València, Spain, Unidad Mixta de Investigación en Genómica y Salud, Fundación para El Fomento de la Investigación Sanit. y Biomédica de la Comunidad Valenciana (FISABIO), Institute for Integrative Systems Biology, Universitat de València, València, Spain

Facilitators :
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Total pages:
1
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Abstract:

Insect lineages feeding on nutritionally restricted diets such as phloem sap, xylem sap, or blood, were able to diversify by acquiring bacterial species that complement lacking nutrients. These bacteria, considered obligate/primary endosymbionts, share a long evolutionary history with their hosts. In some cases, however, these endosymbionts are not able to fulfill all of their host's nutritional requirements, driving the acquisition of additional symbiotic species. Phloem-feeding members of the insect family Aleyrodidae (whiteflies) established an obligate relationship with Candidatus Portiera aleyrodidarum, which provides its hots with essential amino acids and carotenoids. In addition, many whitefly species harbor additional endosymbionts which may potentially further supplement their host's diet. To test this hypothesis, genomes of several endosymbionts of the whiteflies Aleurodicus dispersus, Aleurodicus floccissimus and Trialeurodes vaporariorum were analyzed. In addition to Portiera, all three species were found to harbor one Arsenophonus and one Wolbachia endosymbiont. A comparative analysis of Arsenophonus genomes revealed that although all three are capable of synthesizing B vitamins and cofactors, such as pyridoxal, riboflavin, or folate, their genomes and phylogenetic relationship vary greatly. Arsenophonus of A. floccissimus and T. vaporariorum belong to the same clade, and display characteristics of facultative endosymbionts, such as large genomes (3 Mb) with thousands of genes and pseudogenes, intermediate GC content, and mobile genetic elements. In contrast, Arsenophonus of A. dispersus belongs to a different lineage and displays the characteristics of a primary endosymbiont - a reduced genome (670 kb) with ~400 genes, 32% GC content, and no mobile genetic elements. However, the presence of 274 pseudogenes suggests that this symbiotic association is more recent than other reported primary endosymbionts of hemipterans. The gene repertoire of Arsenophonus of A. dispersus is completely integrated in the symbiotic consortia, and the biosynthesis of most vitamins occurs in shared pathways with its host. In addition, Wolbachia endosymbionts have also retained the ability to produce riboflavin, flavin adenine dinucleotide, and folate, and may make a nutritional contribution. Taken together, our results show that Arsenophonus hold a pivotal place in whitefly nutrition by their ability to produce B vitamins. © 2007 - 2018 Frontiers Media S.A. All Rights Reserved.

Note:
Related Files :
Arsenophonus
Genome reduction
Metabolic complementation
riboflavin
Symbiosis
vitamins
whitefly
Wolbachia
עוד תגיות
תוכן קשור
More details
DOI :
10.3389/fmicb.2018.02254
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
37780
Last updated date:
02/03/2022 17:27
Creation date:
31/10/2018 13:18
Scientific Publication
To B or not to B: Comparative genomics suggests Arsenophonusas a source of B vitamins in whiteflies

Santos-Garcia, D., Department of Entomology, Hebrew University of Jerusalem, Rehovot, Israel; Juravel, K., Department of Entomology, Hebrew University of Jerusalem, Rehovot, Israel;  Latorre, A., Institute for Integrative Systems Biology, Universitat de València-CSIC, València, Spain, Unidad Mixta de Investigación en Genómica y Salud, Fundación para El Fomento de la Investigación Sanit. y Biomédica de la Comunidad Valenciana (FISABIO), Institute for Integrative Systems Biology, Universitat de València, València, Spain; Moya, A., Institute for Integrative Systems Biology, Universitat de València-CSIC, València, Spain, Unidad Mixta de Investigación en Genómica y Salud, Fundación para El Fomento de la Investigación Sanit. y Biomédica de la Comunidad Valenciana (FISABIO), Institute for Integrative Systems Biology, Universitat de València, València, Spain; Morin, S., Department of Entomology, Hebrew University of Jerusalem, Rehovot, Israel; Silva, F.J., Institute for Integrative Systems Biology, Universitat de València-CSIC, València, Spain, Unidad Mixta de Investigación en Genómica y Salud, Fundación para El Fomento de la Investigación Sanit. y Biomédica de la Comunidad Valenciana (FISABIO), Institute for Integrative Systems Biology, Universitat de València, València, Spain

To B or not to B: Comparative genomics suggests Arsenophonusas a source of B vitamins in whiteflies

Insect lineages feeding on nutritionally restricted diets such as phloem sap, xylem sap, or blood, were able to diversify by acquiring bacterial species that complement lacking nutrients. These bacteria, considered obligate/primary endosymbionts, share a long evolutionary history with their hosts. In some cases, however, these endosymbionts are not able to fulfill all of their host's nutritional requirements, driving the acquisition of additional symbiotic species. Phloem-feeding members of the insect family Aleyrodidae (whiteflies) established an obligate relationship with Candidatus Portiera aleyrodidarum, which provides its hots with essential amino acids and carotenoids. In addition, many whitefly species harbor additional endosymbionts which may potentially further supplement their host's diet. To test this hypothesis, genomes of several endosymbionts of the whiteflies Aleurodicus dispersus, Aleurodicus floccissimus and Trialeurodes vaporariorum were analyzed. In addition to Portiera, all three species were found to harbor one Arsenophonus and one Wolbachia endosymbiont. A comparative analysis of Arsenophonus genomes revealed that although all three are capable of synthesizing B vitamins and cofactors, such as pyridoxal, riboflavin, or folate, their genomes and phylogenetic relationship vary greatly. Arsenophonus of A. floccissimus and T. vaporariorum belong to the same clade, and display characteristics of facultative endosymbionts, such as large genomes (3 Mb) with thousands of genes and pseudogenes, intermediate GC content, and mobile genetic elements. In contrast, Arsenophonus of A. dispersus belongs to a different lineage and displays the characteristics of a primary endosymbiont - a reduced genome (670 kb) with ~400 genes, 32% GC content, and no mobile genetic elements. However, the presence of 274 pseudogenes suggests that this symbiotic association is more recent than other reported primary endosymbionts of hemipterans. The gene repertoire of Arsenophonus of A. dispersus is completely integrated in the symbiotic consortia, and the biosynthesis of most vitamins occurs in shared pathways with its host. In addition, Wolbachia endosymbionts have also retained the ability to produce riboflavin, flavin adenine dinucleotide, and folate, and may make a nutritional contribution. Taken together, our results show that Arsenophonus hold a pivotal place in whitefly nutrition by their ability to produce B vitamins. © 2007 - 2018 Frontiers Media S.A. All Rights Reserved.

Scientific Publication
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