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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Identifying genes that are involved in the recovery process of the entomopathogenic nematode Heterorhabditis bacteriophora TTO1 strain
Year:
2009
Source of publication :
IOBC/WPRS Bulletin
Authors :
גלזר, איתמר
;
.
מושיוב, ענת
;
.
קולטאי, חננית
;
.
Volume :
45
Co-Authors:
Facilitators :
From page:
337
To page:
340
(
Total pages:
4
)
Abstract:

Characterizing the process of recovery in Heterorhabditis bacteriophora was done by
identifying genes that are putatively involved in this process. For this purpose, a large scale bioassay
for recovery was established and two subtraction libraries of recovered IJs subtracted by arrested IJs
were constructed. Six hundreds expressed sequence tags (ESTs) were sequenced and annotated
resulting in 300 useful ESTs that were compared to the C. elegans Wormbase and categorized into
functional categories according to gene ontology. Of these, twenty three genes were chosen for further
analysis. These genes were examined for their expression in the recovery process by quantitative (q)
RT-PCR. The results of the RT-qPCR supported the results obtained from the subtraction libraries.
Further analysis to these genes is being done by RNAi-based functional analysis in H. bacteriophora.

Note:
Related Files :
biological control
Entomopathogenic nematodes
Genetics
Heterorhabditis bacteriophora
Nematoda
plant protection
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר מתוך כינוס
;
.
Language:
אנגלית
Editors' remarks:
ID:
37781
Last updated date:
02/03/2022 17:27
Creation date:
31/10/2018 13:37
Scientific Publication
Identifying genes that are involved in the recovery process of the entomopathogenic nematode Heterorhabditis bacteriophora TTO1 strain
45

Characterizing the process of recovery in Heterorhabditis bacteriophora was done by
identifying genes that are putatively involved in this process. For this purpose, a large scale bioassay
for recovery was established and two subtraction libraries of recovered IJs subtracted by arrested IJs
were constructed. Six hundreds expressed sequence tags (ESTs) were sequenced and annotated
resulting in 300 useful ESTs that were compared to the C. elegans Wormbase and categorized into
functional categories according to gene ontology. Of these, twenty three genes were chosen for further
analysis. These genes were examined for their expression in the recovery process by quantitative (q)
RT-PCR. The results of the RT-qPCR supported the results obtained from the subtraction libraries.
Further analysis to these genes is being done by RNAi-based functional analysis in H. bacteriophora.

Scientific Publication
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