Under natural conditions entomopathogenic nematodes (EPNs) are exposed to extreme environmental stresses such as desiccation and heat. Only little is known on the molecular mechanism of stress tolerance in EPNs. Since an adaptation period is needed for acquisition of the anhydrobiotic state and survival at high temperatures, we have focused in our research on the adaptation period of heat and desiccation stresses, affecting nematode survival. We have characterized the heat and desiccation tolerance of selected Steinernema species and performed a whole transcriptome analysis. Our research is expected to provide a better understanding of the biological pathways and molecular mechanisms of EPNs heat and desiccation stress response. This is the first report of the next generation sequencing of EPNs.
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Under natural conditions entomopathogenic nematodes (EPNs) are exposed to extreme environmental stresses such as desiccation and heat. Only little is known on the molecular mechanism of stress tolerance in EPNs. Since an adaptation period is needed for acquisition of the anhydrobiotic state and survival at high temperatures, we have focused in our research on the adaptation period of heat and desiccation stresses, affecting nematode survival. We have characterized the heat and desiccation tolerance of selected Steinernema species and performed a whole transcriptome analysis. Our research is expected to provide a better understanding of the biological pathways and molecular mechanisms of EPNs heat and desiccation stress response. This is the first report of the next generation sequencing of EPNs.