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פותח על ידי קלירמאש פתרונות בע"מ -
Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
Year:
2019
Source of publication :
Postharvest Biology and Technology
Authors :
דרובי, סמיר
;
.
לוין, ילנה
;
.
פייגנברג, אולג
;
.
קישורה, אמית
;
.
רפאל, גינת
;
.
Volume :
149
Co-Authors:

Ballester, A.R., Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Avda. Agustin Escardino, Paterna, Valencia, Spain; Liu, Y., School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China; Norelli, J., Appalachian Fruit Research Station, USDA-ARS, Wiltshire Road, Kearneysville, WV, United States; Gonzalez-Candelas, L., Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Avda. Agustin Escardino, Paterna, Valencia, Spain; Wisniewski, M., Appalachian Fruit Research Station, USDA-ARS, Wiltshire Road, Kearneysville, WV, United Statesl

Facilitators :
From page:
209
To page:
220
(
Total pages:
12
)
Abstract:

Blue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum predicted secretome to reveal potential genes that have a role in host-pathogen interaction. A prediction pipeline was designed using an approach that combines common effector features, transcriptomic data and homology to proteins reported to be involved in pathogenicity of other pytopathogenic fungi. Among 297 genes predicted in P. expansum secretome, 103 genes (35%) were found to code for hydrolytic enzymes. The majority of the secreted enzymes are carbohydrate-degrading enzymes among which five coding for pectin-degrading enzymes are highly induced during the infection and decay of apple fruit by P. expansum, indicating that they may represent an important aspect of pathogenicity and virulence. Applying the pipeline we have predicted 17 candidate genes coding for proteins that are most likely involved in pathogenicity and virulence. One of the top candidates is a subtilisin-related peptidase, S8 (PePRT), proteolytic enzyme highly expressed in planta, and potentially involved in authophagy process. Deletion of PePRT-coding gene resulted in reduced virulence of P. expansum on apples. Moreover, ΔPeprt exhibited decreased sporulation as well as affected mycelial morphology and internal mycelial cell structure. © 2018

Note:
Related Files :
Authophagy
host-pathogen interaction
Host-Pathogen Interactions
Penicillium expansum
Secretome
virulence
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.postharvbio.2018.10.011
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
38687
Last updated date:
02/03/2022 17:27
Creation date:
25/12/2018 09:28
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Scientific Publication
Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples
149

Ballester, A.R., Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Avda. Agustin Escardino, Paterna, Valencia, Spain; Liu, Y., School of Biotechnology and Food Engineering, Hefei University of Technology, Hefei, China; Norelli, J., Appalachian Fruit Research Station, USDA-ARS, Wiltshire Road, Kearneysville, WV, United States; Gonzalez-Candelas, L., Instituto de Agroquímica y Tecnología de Alimentos (IATA-CSIC), Avda. Agustin Escardino, Paterna, Valencia, Spain; Wisniewski, M., Appalachian Fruit Research Station, USDA-ARS, Wiltshire Road, Kearneysville, WV, United Statesl

Identification of pathogenicity-related genes and the role of a subtilisin-related peptidase S8 (PePRT) in authophagy and virulence of Penicillium expansum on apples

Blue mold caused by Penicillium expansum is a major postharvest disease of pome fruit. Several mechanisms possibly involved in P. expansum pathogenicity and virulence. However, factors that mediate pathogenicity and virulence are largely not yet characterized. In this work we analyzed P. expansum predicted secretome to reveal potential genes that have a role in host-pathogen interaction. A prediction pipeline was designed using an approach that combines common effector features, transcriptomic data and homology to proteins reported to be involved in pathogenicity of other pytopathogenic fungi. Among 297 genes predicted in P. expansum secretome, 103 genes (35%) were found to code for hydrolytic enzymes. The majority of the secreted enzymes are carbohydrate-degrading enzymes among which five coding for pectin-degrading enzymes are highly induced during the infection and decay of apple fruit by P. expansum, indicating that they may represent an important aspect of pathogenicity and virulence. Applying the pipeline we have predicted 17 candidate genes coding for proteins that are most likely involved in pathogenicity and virulence. One of the top candidates is a subtilisin-related peptidase, S8 (PePRT), proteolytic enzyme highly expressed in planta, and potentially involved in authophagy process. Deletion of PePRT-coding gene resulted in reduced virulence of P. expansum on apples. Moreover, ΔPeprt exhibited decreased sporulation as well as affected mycelial morphology and internal mycelial cell structure. © 2018

Scientific Publication
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