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Wheat germ agglutinin bound to the outer cuticle of the seed gall nematodes Anguina agrostis and A. tritici
Year:
1991
Source of publication :
Journal of Nematology
Authors :
סלומון, רפי
;
.
שפיגל, יצחק
;
.
Volume :
23
Co-Authors:

Michael A. McClure, Itzhak Kahane, Walter M. Robertson

Facilitators :
From page:
451
To page:
456
(
Total pages:
6
)
Abstract:

The presence of wheat germ agglutinin (WGA) on the cuticular surface of the seed gall nematodes Anguina agrostis and Anguina tritici was demonstrated, and the nature of its binding was examined. Crude extracts from the cuticles of A. tritici agglutinated human red blood cells, and only N-acetylglucosamine (GlucNAc) inhibited the agglutination. Distribution of the lectin was visualized by treating live infective juveniles (J2) with rabbit anti-WGA antibody and staining with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG. The lectin bound to the outer cuticular surface of the whole body wall. Pretreatment with GlucNAc oligomers did not reduce the fluorescence created by the anti-WGA-WGA binding, indicating at least a partial nonspeciflc adhesion of the WGA to the nematode surface. Proteolytic enzyme pretreatments diminished the fluorescence, whereas lipase and periodate pretreatments increased the fluorescence. Adult females and males were labeled only on the head and tail, whereas eggs were not labeled at all. It was concluded that the WGA on the J2 cuticle originates from the host.

Note:
Related Files :
Anguina
Anguina agrostis
Anguina tritici
cuticle
lectin
Nematoda
nematode
wheat germ agglutinin
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
38949
Last updated date:
02/03/2022 17:27
Creation date:
10/01/2019 10:59
Scientific Publication
Wheat germ agglutinin bound to the outer cuticle of the seed gall nematodes Anguina agrostis and A. tritici
23

Michael A. McClure, Itzhak Kahane, Walter M. Robertson

Wheat germ agglutinin bound to the outer cuticle of the seed gall nematodes Anguina agrostis and A. tritici

The presence of wheat germ agglutinin (WGA) on the cuticular surface of the seed gall nematodes Anguina agrostis and Anguina tritici was demonstrated, and the nature of its binding was examined. Crude extracts from the cuticles of A. tritici agglutinated human red blood cells, and only N-acetylglucosamine (GlucNAc) inhibited the agglutination. Distribution of the lectin was visualized by treating live infective juveniles (J2) with rabbit anti-WGA antibody and staining with fluorescein isothiocyanate (FITC)-conjugated goat anti-rabbit IgG. The lectin bound to the outer cuticular surface of the whole body wall. Pretreatment with GlucNAc oligomers did not reduce the fluorescence created by the anti-WGA-WGA binding, indicating at least a partial nonspeciflc adhesion of the WGA to the nematode surface. Proteolytic enzyme pretreatments diminished the fluorescence, whereas lipase and periodate pretreatments increased the fluorescence. Adult females and males were labeled only on the head and tail, whereas eggs were not labeled at all. It was concluded that the WGA on the J2 cuticle originates from the host.

Scientific Publication
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