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פותח על ידי קלירמאש פתרונות בע"מ -
Proteomic Analysis of the Resistance Mechanisms in Sugarcane during Sporisorium scitamineum Infection
Year:
2019
Authors :
סולנקי, מנוג' קומאר
;
.
Volume :
20
Co-Authors:

Singh, P., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Song, Q.-Q., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Singh, R.K., Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Li, H.-B., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Malviya, M.K., Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China. mukeshmicro@rediffmail.com; Verma, K.K., Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Yang, L.-T., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Li, Y.-R., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Facilitators :
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Abstract:

Smut disease is caused by Sporisorium scitamineum, an important sugarcane fungal pathogen causing an extensive loss in yield and sugar quality. The available literature suggests that there are two types of smut resistance mechanisms: external resistance by physical or chemical barriers and intrinsic internal resistance mechanisms operating at host⁻pathogen interaction at cellular and molecular levels. The nature of smut resistance mechanisms, however, remains largely unknown. The present study investigated the changes in proteome occurring in two sugarcane varieties with contrasting susceptibility to smut-F134 and NCo310-at whip development stage after S. scitamineum infection. Total proteins from pathogen inoculated and uninoculated (control) leaves were separated by two-dimensional gel electrophoresis (2D-PAGE). Protein identification was performed using BLASTp and tBLASTn against NCBI nonredundant protein databases and EST databases, respectively. A total of thirty proteins spots representing differentially expressed proteins (DEPs), 16 from F134 and 14 from NCo310, were identified and analyzed by MALDI-TOF/TOF MS. In F134, 4 DEPs were upregulated and nine were downregulated, while, nine were upregulated and three were downregulated in NCo310. The DEPs were associated with DNA binding, metabolic processes, defense, stress response, photorespiration, protein refolding, chloroplast, nucleus and plasma membrane. Finally, the expression of CAT, SOD, and PAL with recognized roles in S. scitamineum infection in both sugarcane verities were analyzed by real-time quantitative PCR (RT-qPCR) technique. Identification of genes critical for smut resistance in sugarcane will increase our knowledge of S. scitamineum-sugarcane interaction and help to develop molecular and conventional breeding strategies for variety improvement.

Note:
Related Files :
ISR
proteomics
RT-qPCR
smut
Sporisorium scitamineum
sugarcane
עוד תגיות
תוכן קשור
More details
DOI :
10.3390/ijms20030569
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
39308
Last updated date:
02/03/2022 17:27
Creation date:
12/02/2019 10:18
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Scientific Publication
Proteomic Analysis of the Resistance Mechanisms in Sugarcane during Sporisorium scitamineum Infection
20

Singh, P., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Song, Q.-Q., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Singh, R.K., Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Li, H.-B., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Malviya, M.K., Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China. mukeshmicro@rediffmail.com; Verma, K.K., Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China; Yang, L.-T., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China; Li, Y.-R., Agricultural College, State Key Laboratory of Subtropical Bioresources Conservation and Utilization, Guangxi University, Nanning, 530005, China, Guangxi Key Laboratory of Sugarcane Genetic Improvement, Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture, Sugarcane Research Center, Chinese Academy of Agricultural Sciences; Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Proteomic Analysis of the Resistance Mechanisms in Sugarcane during Sporisorium scitamineum Infection

Smut disease is caused by Sporisorium scitamineum, an important sugarcane fungal pathogen causing an extensive loss in yield and sugar quality. The available literature suggests that there are two types of smut resistance mechanisms: external resistance by physical or chemical barriers and intrinsic internal resistance mechanisms operating at host⁻pathogen interaction at cellular and molecular levels. The nature of smut resistance mechanisms, however, remains largely unknown. The present study investigated the changes in proteome occurring in two sugarcane varieties with contrasting susceptibility to smut-F134 and NCo310-at whip development stage after S. scitamineum infection. Total proteins from pathogen inoculated and uninoculated (control) leaves were separated by two-dimensional gel electrophoresis (2D-PAGE). Protein identification was performed using BLASTp and tBLASTn against NCBI nonredundant protein databases and EST databases, respectively. A total of thirty proteins spots representing differentially expressed proteins (DEPs), 16 from F134 and 14 from NCo310, were identified and analyzed by MALDI-TOF/TOF MS. In F134, 4 DEPs were upregulated and nine were downregulated, while, nine were upregulated and three were downregulated in NCo310. The DEPs were associated with DNA binding, metabolic processes, defense, stress response, photorespiration, protein refolding, chloroplast, nucleus and plasma membrane. Finally, the expression of CAT, SOD, and PAL with recognized roles in S. scitamineum infection in both sugarcane verities were analyzed by real-time quantitative PCR (RT-qPCR) technique. Identification of genes critical for smut resistance in sugarcane will increase our knowledge of S. scitamineum-sugarcane interaction and help to develop molecular and conventional breeding strategies for variety improvement.

Scientific Publication
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