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In plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids
Year:
2019
Source of publication :
Nature Microbiology
Authors :
זיו, כרמית
;
.
Volume :
4
Co-Authors:

Schleyer, G., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Shahaf, N., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel;  Dong, Y., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Meoded, R.A., Institute of Microbiology, ETH Zurich, Zurich, Switzerland; Helfrich, E.J.N., Institute of Microbiology, ETH Zurich, Zurich, Switzerland; Schatz, D., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Rosenwasser, S., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel, Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel; Rogachev, I., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Aharoni, A., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Piel, J., Institute of Microbiology, ETH Zurich, Zurich, Switzerland; Vardi, A., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel

Facilitators :
From page:
527
To page:
538
(
Total pages:
12
)
Abstract:

Tapping into the metabolic crosstalk between a host and its virus can reveal unique strategies employed during infection. Viral infection is a dynamic process that generates an evolving metabolic landscape. Gaining a continuous view into the infection process is highly challenging and is limited by current metabolomics approaches, which typically measure the average of the entire population at various stages of infection. Here, we took an innovative approach to study the metabolic basis of host–virus interactions between the bloom-forming alga Emiliania huxleyi and its specific virus. We combined a classical method in virology, the plaque assay, with advanced mass spectrometry imaging (MSI), an approach we termed ‘in plaque-MSI’. Taking advantage of the spatial characteristics of the plaque, we mapped the metabolic landscape induced during infection in a high spatiotemporal resolution, unfolding the infection process in a continuous manner. Further unsupervised spatially aware clustering, combined with known lipid biomarkers, revealed a systematic metabolic shift during infection towards lipids containing the odd-chain fatty acid pentadecanoic acid (C15:0). Applying ‘in plaque-MSI’ may facilitate the discovery of bioactive compounds that mediate the chemical arms race of host–virus interactions in diverse model systems. © 2019, The Author(s), under exclusive licence to Springer Nature Limited.

Note:
Related Files :
fatty acid
Lipids
Spectrometry
Viral infection
עוד תגיות
תוכן קשור
More details
DOI :
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
39369
Last updated date:
02/03/2022 17:27
Creation date:
20/02/2019 10:54
Scientific Publication
In plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids
4

Schleyer, G., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Shahaf, N., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel;  Dong, Y., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Meoded, R.A., Institute of Microbiology, ETH Zurich, Zurich, Switzerland; Helfrich, E.J.N., Institute of Microbiology, ETH Zurich, Zurich, Switzerland; Schatz, D., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Rosenwasser, S., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel, Institute of Plant Sciences and Genetics in Agriculture, The Hebrew University of Jerusalem, Rehovot, Israel; Rogachev, I., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Aharoni, A., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel; Piel, J., Institute of Microbiology, ETH Zurich, Zurich, Switzerland; Vardi, A., Department of Plant and Environmental Sciences, Weizmann Institute of Science, Rehovot, Israel

In plaque-mass spectrometry imaging of a bloom-forming alga during viral infection reveals a metabolic shift towards odd-chain fatty acid lipids

Tapping into the metabolic crosstalk between a host and its virus can reveal unique strategies employed during infection. Viral infection is a dynamic process that generates an evolving metabolic landscape. Gaining a continuous view into the infection process is highly challenging and is limited by current metabolomics approaches, which typically measure the average of the entire population at various stages of infection. Here, we took an innovative approach to study the metabolic basis of host–virus interactions between the bloom-forming alga Emiliania huxleyi and its specific virus. We combined a classical method in virology, the plaque assay, with advanced mass spectrometry imaging (MSI), an approach we termed ‘in plaque-MSI’. Taking advantage of the spatial characteristics of the plaque, we mapped the metabolic landscape induced during infection in a high spatiotemporal resolution, unfolding the infection process in a continuous manner. Further unsupervised spatially aware clustering, combined with known lipid biomarkers, revealed a systematic metabolic shift during infection towards lipids containing the odd-chain fatty acid pentadecanoic acid (C15:0). Applying ‘in plaque-MSI’ may facilitate the discovery of bioactive compounds that mediate the chemical arms race of host–virus interactions in diverse model systems. © 2019, The Author(s), under exclusive licence to Springer Nature Limited.

Scientific Publication
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