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פותח על ידי קלירמאש פתרונות בע"מ -
Alkaloid chemodiversity in Mandragora spp. is associated with loss-of-functionality of MoH6H, a hyoscyamine 6β-hydroxylase gene
Year:
2019
Source of publication :
Plant Science
Authors :
אורן, אלעד
;
.
דוידוביץ'-רקנאטי, רחל
;
.
דורון-פייגנבאום, עדי
;
.
לוינסון, אפרים
;
.
שלזינגר, דניאל רוברטו
;
.
Volume :
283
Co-Authors:

 Volis, S., Kunming Institute of Botany, 132, Lanhei Road, Kunming, Yunnan  650201, China;  Vendramin, V., IGA Technology Services, Via J. Linussio 51, Udine, 33100, Italy; Cattonaro, F., IGA Technology Services, Via J. Linussio 51, Udine, 33100, Italy; Hooper, M., Cell & Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, Scotland  DD2 5DA, United Kingdom;  Taylor, M., Cell & Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, Scotland  DD2 5DA, United Kingdom; Sitrit, Y., The Jacob Blaustein Institutes f Desert Research, Ben-Gurion University of The Negev, P.O. Box 653, Beer-Sheva, 84105, Israel; Inbar, M., Department of Evolutionary & Environmental Biology, University of Haifa, Mount Carmel, Haifa, 31905, Israel;

Facilitators :
From page:
301
To page:
310
(
Total pages:
10
)
Abstract:

Mandrakes (Mandragora spp., Solanaceae) are known to contain tropane alkaloids and have been used since antiquity in traditional medicine. Tropane alkaloids such as scopolamine and hyoscyamine are used in modern medicine to treat pain, motion sickness, as eye pupil dilators and antidotes against organo-phosphate poisoning. Hyoscyamine is converted to 6β-hydroxyhyoscyamine (anisodamine) and scopolamine by hyoscyamine 6β-hydroxylase (H6H), a 2-oxoglutarate dependent dioxygenase. We describe here a marked chemo-diversity in the tropane alkaloid content in Mandragora spp. M. officinarum and M. turcomanica lack anisodamine and scopolamine but display up to 10 fold higher hyoscyamine levels as compared with M. autumnalis. Transcriptomic analyses revealed that H6H is highly conserved among scopolamine-producing Solanaceae. MoH6H present in M. officinarum differs in several amino acid residues including a homozygotic mutation in the substrate binding region of the protein and its prevalence among accessions was confirmed by Cleaved-Amplified-Polymorphic-Sequence analyses. Functional expression revealed that MaH6H, a gene isolated from M. autumnalis encodes an active H6H enzyme while the MoH6H sequence isolated from M. officinarum was functionally inactive. A single G to T mutation in nucleotide 663 of MoH6H is associated with the lack of anisodamine and scopolamine in M. officinalis. © 2019 Elsevier B.V.

Note:
Related Files :
CAPS
Solanaceae
Tropane alkaloids
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.plantsci.2019.03.013
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
39819
Last updated date:
02/03/2022 17:27
Creation date:
03/04/2019 11:38
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Scientific Publication
Alkaloid chemodiversity in Mandragora spp. is associated with loss-of-functionality of MoH6H, a hyoscyamine 6β-hydroxylase gene
283

 Volis, S., Kunming Institute of Botany, 132, Lanhei Road, Kunming, Yunnan  650201, China;  Vendramin, V., IGA Technology Services, Via J. Linussio 51, Udine, 33100, Italy; Cattonaro, F., IGA Technology Services, Via J. Linussio 51, Udine, 33100, Italy; Hooper, M., Cell & Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, Scotland  DD2 5DA, United Kingdom;  Taylor, M., Cell & Molecular Sciences, The James Hutton Institute, Invergowrie, Dundee, Scotland  DD2 5DA, United Kingdom; Sitrit, Y., The Jacob Blaustein Institutes f Desert Research, Ben-Gurion University of The Negev, P.O. Box 653, Beer-Sheva, 84105, Israel; Inbar, M., Department of Evolutionary & Environmental Biology, University of Haifa, Mount Carmel, Haifa, 31905, Israel;

Alkaloid chemodiversity in Mandragora spp. is associated with loss-of-functionality of MoH6H, a hyoscyamine 6β-hydroxylase gene

Mandrakes (Mandragora spp., Solanaceae) are known to contain tropane alkaloids and have been used since antiquity in traditional medicine. Tropane alkaloids such as scopolamine and hyoscyamine are used in modern medicine to treat pain, motion sickness, as eye pupil dilators and antidotes against organo-phosphate poisoning. Hyoscyamine is converted to 6β-hydroxyhyoscyamine (anisodamine) and scopolamine by hyoscyamine 6β-hydroxylase (H6H), a 2-oxoglutarate dependent dioxygenase. We describe here a marked chemo-diversity in the tropane alkaloid content in Mandragora spp. M. officinarum and M. turcomanica lack anisodamine and scopolamine but display up to 10 fold higher hyoscyamine levels as compared with M. autumnalis. Transcriptomic analyses revealed that H6H is highly conserved among scopolamine-producing Solanaceae. MoH6H present in M. officinarum differs in several amino acid residues including a homozygotic mutation in the substrate binding region of the protein and its prevalence among accessions was confirmed by Cleaved-Amplified-Polymorphic-Sequence analyses. Functional expression revealed that MaH6H, a gene isolated from M. autumnalis encodes an active H6H enzyme while the MoH6H sequence isolated from M. officinarum was functionally inactive. A single G to T mutation in nucleotide 663 of MoH6H is associated with the lack of anisodamine and scopolamine in M. officinalis. © 2019 Elsevier B.V.

Scientific Publication
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