נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Visualizing embolism propagation in gas-injected leaves
Year:
2019
Authors :
הוכברג, אורי
;
.
Volume :
Co-Authors:

Alexandre Ponomarenko, - Department of Organismic and Evolutionary Biology, Harvard University CITY: Cambridge STATE: Massachusetts 02138 USA

Yong-Jiang Zhang, N. Michele Holbrook , Fulton E Rockwell    -  Harvard University CITY: Cambridge STATE: Massachusetts 02138 USA

 

 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Since the xylem in leaves is thought to be at the greatest risk of cavitation, reliable and efficient methods to characterize leaf xylem vulnerability are of interest. We report a method to generate leaf xylem vulnerability curves (VCs) by gas injection. Using optical light transmission, we visualized embolism propagation in grapevine (Vitis vinifera) and red oak (Quercus rubra L.) leaves injected with positive gas pressure. This resulted in a rapid, stepwise reduction of transmitted light, identical to that observed during leaf dehydration, confirming that the optical method detects gas bubbles and provides insights into the air-seeding hypothesis. In red oak, xylem VCs generated using gas injection were similar to those generated using bench dehydration, but indicated 50% loss of conductivity at lower tension (~0.4 MPa) in grapevine. In determining VC, this method eliminates the need to ascertain xylem tension, thus avoiding potential errors in water potential estimations. It is also much faster (1 hour per VC). However, severing the petiole and applying high-pressure gas could affect air-seeding and the generated VC. We discuss potential artifacts arising from gas injection and recommend comparison of this method with a more standard procedure before it is assumed to be suitable for a given species.

Note:

We are delighted to let you know that researcher Uri Hochberg from Volcani Center recently published an article in Plant Physiology which was one of our top 5 articles during the month of March 2019 based on Altmetrics.  Altmetrics is a measure of how much attention journal articles are receiving based on discussions on the web, research blogs, social media, Wikipedia and in public policy documents and the like.

Linda J. Palmer

Institutional Subscriptions

American Society of Plant Biologists

THE PLANT CELL and PLANT PHYSIOLOGY

Related Files :
cavitation
gas
injection
leaves
Quercus robur
Vitis vinifera
xylem
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1104/pp.18.01284
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
40080
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2019 09:02
You may also be interested in
Scientific Publication
Visualizing embolism propagation in gas-injected leaves

Alexandre Ponomarenko, - Department of Organismic and Evolutionary Biology, Harvard University CITY: Cambridge STATE: Massachusetts 02138 USA

Yong-Jiang Zhang, N. Michele Holbrook , Fulton E Rockwell    -  Harvard University CITY: Cambridge STATE: Massachusetts 02138 USA

 

 

Visualizing embolism propagation in gas-injected leaves

Since the xylem in leaves is thought to be at the greatest risk of cavitation, reliable and efficient methods to characterize leaf xylem vulnerability are of interest. We report a method to generate leaf xylem vulnerability curves (VCs) by gas injection. Using optical light transmission, we visualized embolism propagation in grapevine (Vitis vinifera) and red oak (Quercus rubra L.) leaves injected with positive gas pressure. This resulted in a rapid, stepwise reduction of transmitted light, identical to that observed during leaf dehydration, confirming that the optical method detects gas bubbles and provides insights into the air-seeding hypothesis. In red oak, xylem VCs generated using gas injection were similar to those generated using bench dehydration, but indicated 50% loss of conductivity at lower tension (~0.4 MPa) in grapevine. In determining VC, this method eliminates the need to ascertain xylem tension, thus avoiding potential errors in water potential estimations. It is also much faster (1 hour per VC). However, severing the petiole and applying high-pressure gas could affect air-seeding and the generated VC. We discuss potential artifacts arising from gas injection and recommend comparison of this method with a more standard procedure before it is assumed to be suitable for a given species.

We are delighted to let you know that researcher Uri Hochberg from Volcani Center recently published an article in Plant Physiology which was one of our top 5 articles during the month of March 2019 based on Altmetrics.  Altmetrics is a measure of how much attention journal articles are receiving based on discussions on the web, research blogs, social media, Wikipedia and in public policy documents and the like.

Linda J. Palmer

Institutional Subscriptions

American Society of Plant Biologists

THE PLANT CELL and PLANT PHYSIOLOGY

Scientific Publication
You may also be interested in