נגישות
menu      
חיפוש מתקדם
תחביר
חפש...
הספר "אוצר וולקני"
אודות
תנאי שימוש
ניהול
קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
The zinc-finger transcription factor CcLOL1 controls chloroplast development and immature pepper fruit color in Capsicum chinense and its function is conserved in tomato
Year:
2019
Source of publication :
Plant Journal
Authors :
בורובסקי, ילנה
;
.
דורון-פייגנבאום, עדי
;
.
מוהאן, ויג'י
;
.
מונסונגו, נועם
;
.
פארן, אילן
;
.
קמארה, יצחק
;
.
שבתאי, שרה
;
.
Volume :
99
Co-Authors:

Hill, T., Seed Biotechnology Center, University of California, DavisCA, United States; Chen, S., Seed Biotechnology Center, University of California, DavisCA, United States; Stoffel, K., Seed Biotechnology Center, University of California, DavisCA, United States; Van Deynze, A., Seed Biotechnology Center, University of California, DavisCA, United States;

Facilitators :
From page:
41
To page:
55
(
Total pages:
15
)
Abstract:

Chloroplast development and chlorophyll content in the immature fruit has a major impact on the morphology and quality in pepper (Capsicum spp.) fruit. Two major quantitative trait loci (QTLs), pc1 and pc10 that affect chlorophyll content in the pepper fruit by modulation of chloroplast compartment size were previously identified in chromosomes 1 and 10, respectively. The pepper homolog of GOLDEN2-LIKE transcription factor (CaGLK2) has been found as underlying pc10, similar to its effect on tomato chloroplast development. In the present study, we identified the pepper homolog of the zinc-finger transcription factor LOL1 (LSD ONE LIKE1; CcLOL1) as the gene underlying pc1. LOL1 has been identified in Arabidopsis as a positive regulator of programmed cell death and we report here on its role in controlling fruit development in the Solanaceae in a fruit-specific manner. The light-green C. chinense parent used for QTL mapping was found to carry a null mutation in CcLOL1. Verification of the function of the gene was done by generating CRISPR/Cas9 knockout mutants of the orthologous tomato gene resulting in light-green tomato fruits, indicating functional conservation of the orthologous genes in controlling chlorophyll content in the Solanaceae. Transcriptome profiling of light and dark-green bulks differing for pc1, showed that the QTL affects multiple photosynthesis and oxidation-reduction associated genes in the immature green fruit. Allelic diversity of three known genes CcLOL1, CaGLK2, and CcAPRR2 that influence pepper immature fruit color, was found to be associated with variation in chlorophyll content primarily in C. chinense. © 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd

Note:
Related Files :
Capsicum spp
chlorophyll
Chloroplast development
Plants
transcription factor
Zinc
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/tpj.14305
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
40108
Last updated date:
02/03/2022 17:27
Creation date:
16/04/2019 13:30
You may also be interested in
Scientific Publication
The zinc-finger transcription factor CcLOL1 controls chloroplast development and immature pepper fruit color in Capsicum chinense and its function is conserved in tomato
99

Hill, T., Seed Biotechnology Center, University of California, DavisCA, United States; Chen, S., Seed Biotechnology Center, University of California, DavisCA, United States; Stoffel, K., Seed Biotechnology Center, University of California, DavisCA, United States; Van Deynze, A., Seed Biotechnology Center, University of California, DavisCA, United States;

The zinc-finger transcription factor CcLOL1 controls chloroplast development and immature pepper fruit color in Capsicum chinense and its function is conserved in tomato

Chloroplast development and chlorophyll content in the immature fruit has a major impact on the morphology and quality in pepper (Capsicum spp.) fruit. Two major quantitative trait loci (QTLs), pc1 and pc10 that affect chlorophyll content in the pepper fruit by modulation of chloroplast compartment size were previously identified in chromosomes 1 and 10, respectively. The pepper homolog of GOLDEN2-LIKE transcription factor (CaGLK2) has been found as underlying pc10, similar to its effect on tomato chloroplast development. In the present study, we identified the pepper homolog of the zinc-finger transcription factor LOL1 (LSD ONE LIKE1; CcLOL1) as the gene underlying pc1. LOL1 has been identified in Arabidopsis as a positive regulator of programmed cell death and we report here on its role in controlling fruit development in the Solanaceae in a fruit-specific manner. The light-green C. chinense parent used for QTL mapping was found to carry a null mutation in CcLOL1. Verification of the function of the gene was done by generating CRISPR/Cas9 knockout mutants of the orthologous tomato gene resulting in light-green tomato fruits, indicating functional conservation of the orthologous genes in controlling chlorophyll content in the Solanaceae. Transcriptome profiling of light and dark-green bulks differing for pc1, showed that the QTL affects multiple photosynthesis and oxidation-reduction associated genes in the immature green fruit. Allelic diversity of three known genes CcLOL1, CaGLK2, and CcAPRR2 that influence pepper immature fruit color, was found to be associated with variation in chlorophyll content primarily in C. chinense. © 2019 The Authors The Plant Journal © 2019 John Wiley & Sons Ltd

Scientific Publication
You may also be interested in