חיפוש מתקדם
New Phytologist
  • Emission of volatiles at advanced stages of flower development is a strategy used by plants to lure pollinators to the flower. We reveal that GA negatively regulates floral scent production in petunia.
  • We used Agrobacterium‐mediated transient expression of GA‐20ox in petunia flowers and a virus‐induced gene silencing approach to knock down DELLA expression, measured volatile emission, internal pool sizes and GA levels by GC‐MS or LC–MS/MS, and analyzed transcript levels of scent‐related phenylpropanoid‐pathway genes.
  • We show that GA has a negative effect on the concentrations of accumulated and emitted phenylpropanoid volatiles in petunia flowers; this effect is exerted through transcriptional/post‐transcriptional downregulation of regulatory and biosynthetic scent‐related genes. Both overexpression of GA20‐ox, a GA‐biosynthesis gene, and suppression of DELLA, a repressor of GA‐signal transduction, corroborated GA's negative regulation of floral scent.
  • We present a model in which GA‐dependent timing of the sequential activation of different branches of the phenylpropanoid pathway during flower development may represent a link between the showy traits controlling pollinator attraction, namely color and scent.
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הספר "אוצר וולקני"
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תנאי שימוש
GA as a regulatory link between the showy floral traits color and scent

Jasmin Ravid , Yumiko Takebayashi, Mitsunori Seo, Alon Cna'ani, Javiera Aravena‐Calvo , Tania Masci , Moran Farhi , Alexander Vainstein

GA as a regulatory link between the showy floral traits color and scent .
  • Emission of volatiles at advanced stages of flower development is a strategy used by plants to lure pollinators to the flower. We reveal that GA negatively regulates floral scent production in petunia.
  • We used Agrobacterium‐mediated transient expression of GA‐20ox in petunia flowers and a virus‐induced gene silencing approach to knock down DELLA expression, measured volatile emission, internal pool sizes and GA levels by GC‐MS or LC–MS/MS, and analyzed transcript levels of scent‐related phenylpropanoid‐pathway genes.
  • We show that GA has a negative effect on the concentrations of accumulated and emitted phenylpropanoid volatiles in petunia flowers; this effect is exerted through transcriptional/post‐transcriptional downregulation of regulatory and biosynthetic scent‐related genes. Both overexpression of GA20‐ox, a GA‐biosynthesis gene, and suppression of DELLA, a repressor of GA‐signal transduction, corroborated GA's negative regulation of floral scent.
  • We present a model in which GA‐dependent timing of the sequential activation of different branches of the phenylpropanoid pathway during flower development may represent a link between the showy traits controlling pollinator attraction, namely color and scent.
Scientific Publication
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