חיפוש מתקדם
Virus-induced gene silencing
  • Alon Cna’ani, Alexander Vainstein

Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting.

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תנאי שימוש
Virus-Aided Gene Expression and Silencing Using TRV for Functional Analysis of Floral Scent-Related Ge -Abstract
  • Alon Cna’ani, Alexander Vainstein
Virus-Aided Gene Expression and Silencing Using TRV for Functional Analysis of Floral Scent-Related Ge

Flower scent is a composite character determined by a complex mixture of low-molecular-weight volatile molecules. Despite the importance of floral fragrance, our knowledge on factors regulating these pathways remains sketchy. Virus-induced gene silencing (VIGS) and virus-aided gene expression (VAGE) are characterized by a simple inoculation procedure and rapid results as compared to transgenesis, allowing screening and characterization of scent-related genes. Here, we describe methods using TRV as a VIGS/VAGE vector for the characterization of scent-related genes, protein compartmentalization studies, and protein subcellular targeting.

Scientific Publication
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