חיפוש מתקדם

Lyu, L., College of Plant Protection, Gansu Agricultural University, Lanzhou, 730070, China; Bi, Y., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China; Li, S., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China; Xue, H., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China; Zhang, Z., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China;

Mitochondria play an essential part in fighting against pathogen infection in the defense responses of fruits. In this study, we investigated the reactive oxygen species (ROS) production, energy metabolism, and changes of mitochondrial proteins in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum. The results indicated that the fungal infection obviously induced the H 2 O 2 accumulation in mitochondria. Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H + -ATPase, and Ca 2+ -ATPase. However, the activities of Ca 2+ -ATPase and H + -ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi. A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transporter and mitochondria dysfunction. Taken together, our results suggest that mitochondria play crucial roles in the early defense responses of muskmelons against T. roseum infection through regulation of ROS production and energy metabolism. © 2019 American Chemical Society.

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הספר "אוצר וולקני"
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תנאי שימוש
Early Defense Responses Involved in Mitochondrial Energy Metabolism and Reactive Oxygen Species Accumulation in Harvested Muskmelons Infected by Trichothecium roseum
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Lyu, L., College of Plant Protection, Gansu Agricultural University, Lanzhou, 730070, China; Bi, Y., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China; Li, S., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China; Xue, H., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China; Zhang, Z., College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, 730070, China;

Early Defense Responses Involved in Mitochondrial Energy Metabolism and Reactive Oxygen Species Accumulation in Harvested Muskmelons Infected by Trichothecium roseum

Mitochondria play an essential part in fighting against pathogen infection in the defense responses of fruits. In this study, we investigated the reactive oxygen species (ROS) production, energy metabolism, and changes of mitochondrial proteins in harvested muskmelon fruits (Cucumis melo cv. Yujinxiang) inoculated with Trichothecium roseum. The results indicated that the fungal infection obviously induced the H 2 O 2 accumulation in mitochondria. Enzyme activities were inhibited in the first 6 h postinoculation (hpi), including succinic dehydrogenase, cytochrome c oxidase, H + -ATPase, and Ca 2+ -ATPase. However, the activities of Ca 2+ -ATPase and H + -ATPase and the contents of intracellular adenosine triphosphate (ATP) were improved to a higher level at 12 hpi. A total of 42 differentially expressed proteins were identified through tandem mass tags-based proteomic analyses, which are mainly involved in energy metabolism, stress responses and redox homeostasis, glycolysis and tricarboxylic acid cycle, and transporter and mitochondria dysfunction. Taken together, our results suggest that mitochondria play crucial roles in the early defense responses of muskmelons against T. roseum infection through regulation of ROS production and energy metabolism. © 2019 American Chemical Society.

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