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The hemolymph of Amblyomma americanum and Dermacentor variabilis was evaluated as a potential material for an antitick vaccine. Hemolymph was collected from replete females and centrifuged to remove the hemocytes. Cell-free hemolymph was used to immunize rabbits. Immunized rabbits developed high antihemolymph antibody titers (ca. l.Oxl06) and had no ill side effects. Rabbits were infested with larvae, nymphs and adults at the same time. The biological performance of ticks fed on immunized rabbits was virtually identical to that of ticks fed on nonimmunized rabbits. Usually, the mean engorgments weights of nymphs and females and the weights of the egg-masses of both species were slightly higher for ticks fed on the nonimmunized rabbits but no significant differences were established due to a large standard deviation. The hemolymph electrophoretic patterns of ticks fed on immunized and nonimmunized rabbits were the same. The possibility of deactivating a single hemolymph component with specific antibodies is discussed.

Submitted to the Faculty of the Graduate College of the Oklahoma State University in partial fulfillment of the requirements for the Degree of DOCTOR OF PHILOSOPHY

פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Evaluation of tick hemolymph as a potential material for an antitick vaccine
Evaluation of tick hemolymph as a potential material for an antitick vaccine

The hemolymph of Amblyomma americanum and Dermacentor variabilis was evaluated as a potential material for an antitick vaccine. Hemolymph was collected from replete females and centrifuged to remove the hemocytes. Cell-free hemolymph was used to immunize rabbits. Immunized rabbits developed high antihemolymph antibody titers (ca. l.Oxl06) and had no ill side effects. Rabbits were infested with larvae, nymphs and adults at the same time. The biological performance of ticks fed on immunized rabbits was virtually identical to that of ticks fed on nonimmunized rabbits. Usually, the mean engorgments weights of nymphs and females and the weights of the egg-masses of both species were slightly higher for ticks fed on the nonimmunized rabbits but no significant differences were established due to a large standard deviation. The hemolymph electrophoretic patterns of ticks fed on immunized and nonimmunized rabbits were the same. The possibility of deactivating a single hemolymph component with specific antibodies is discussed.

Submitted to the Faculty of the Graduate College of the Oklahoma State University in partial fulfillment of the requirements for the Degree of DOCTOR OF PHILOSOPHY

Scientific Publication
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