חיפוש מתקדם
וולקני וויס

Many types of bacteria proliferate in milk, as it provides an ideal growth medium due to its high nutritional value. The opaqueness of  milk does not enable measuring of optical density to  determine bacterial growth, which significantly lengthens analysis of bacterial growth in milk for food safety and basic research purposes. We developed a unique real-time fluorescence based  method for tracking bacterial growth in milk, which applies a microplate reader and direct microscopic observation. A fluorescently-tagged Bacillus subtilis was chosen as a model organism for this assay since this bacterium is ubiquitous in milk and its products. We suggest that the developed assay can be applied for rapid detection of B. subtilis growth in milk.        

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הספר "אוצר וולקני"
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תנאי שימוש
Using fluorescent labeling for tracking Bacillus subtilis growth in milk
6

Many types of bacteria proliferate in milk, as it provides an ideal growth medium due to its high nutritional value. The opaqueness of  milk does not enable measuring of optical density to  determine bacterial growth, which significantly lengthens analysis of bacterial growth in milk for food safety and basic research purposes. We developed a unique real-time fluorescence based  method for tracking bacterial growth in milk, which applies a microplate reader and direct microscopic observation. A fluorescently-tagged Bacillus subtilis was chosen as a model organism for this assay since this bacterium is ubiquitous in milk and its products. We suggest that the developed assay can be applied for rapid detection of B. subtilis growth in milk.        

Scientific Publication
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