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פותח על ידי קלירמאש פתרונות בע"מ -
The multi-allelic APRR2 gene is associated with fruit pigment accumulation in melon and watermelon
Year:
2019
Source of publication :
Journal of Experimental Botany
Authors :
אורן, אלעד
;
.
בורגר, יוסף
;
.
גור, עמית
;
.
דורון-פייגנבאום, עדי
;
.
דפנא, אסף
;
.
וקסלר, לאה
;
.
מאיר, איילה
;
.
פורטנוי, ויטלי
;
.
צורי, גליל
;
.
קניגסוולד, מרב
;
.
קציר, נורית
;
.
שפר, ארתור
;
.
תדמור, יעקב
;
.
Volume :
1; 70(15)
Co-Authors:

Amnon Levi  - United States Department of Agriculture-Agricultural Research Service, US Vegetable Laboratory, Charleston, SC, USA

Edward S Buckler  - Plant Breeding and Genetics Section, Cornell University, Ithaca, NY, USA. United States Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA

 

Facilitators :
From page:
3781
To page:
3794
(
Total pages:
14
)
Abstract:

Color and pigment contents are important aspects of fruit quality and consumer acceptance of cucurbit crops. Here, we describe the independent mapping and cloning of a common causative APRR2 gene regulating pigment accumulation in melon and watermelon. We initially show that the APRR2 transcription factor is causative for the qualitative difference between dark and light green rind in both crops. Further analyses establish the link between sequence or expression level variations in the CmAPRR2 gene and pigment content in the rind and flesh of mature melon fruits. A genome-wide association study (GWAS) of young fruit rind color in a panel composed of 177 diverse melon accessions did not result in any significant association, leading to an earlier assumption that multiple genes are involved in shaping the overall phenotypic variation in this trait. Through resequencing of 25 representative accessions and allelism tests between light rind accessions, we show that multiple independent single nucleotide polymorphisms in the CmAPRR2 gene are causative of the light rind phenotype. The multi-haplotypic nature of this gene explains the lack of detection power obtained through genotyping by sequencing-based GWAS and confirms the pivotal role of this gene in shaping fruit color variation in melon. This study demonstrates the power of combining bi- and multi-allelic designs with deep sequencing, to resolve lack of power due to high haplotypic diversity and low allele frequencies. Due to its central role and broad effect on pigment accumulation in fruits, the APRR2 gene is an attractive target for carotenoid bio-fortification of cucurbit crops

Note:
Related Files :
APRR2
BSA-Seq
carotenoids
chlorophyll
Citrullus lanatus
Fruit quality
GWAS
Melon
QTL
RNA-seq
RNA-Sequencing
עוד תגיות
תוכן קשור
More details
DOI :
10.1093/jxb/erz182
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
43214
Last updated date:
02/03/2022 17:27
Creation date:
19/08/2019 08:34
Scientific Publication
The multi-allelic APRR2 gene is associated with fruit pigment accumulation in melon and watermelon
1; 70(15)

Amnon Levi  - United States Department of Agriculture-Agricultural Research Service, US Vegetable Laboratory, Charleston, SC, USA

Edward S Buckler  - Plant Breeding and Genetics Section, Cornell University, Ithaca, NY, USA. United States Department of Agriculture-Agricultural Research Service, Robert W. Holley Center for Agriculture and Health, Ithaca, NY, USA

 

The multi-allelic APRR2 gene is associated with fruit pigment accumulation in melon and watermelon

Color and pigment contents are important aspects of fruit quality and consumer acceptance of cucurbit crops. Here, we describe the independent mapping and cloning of a common causative APRR2 gene regulating pigment accumulation in melon and watermelon. We initially show that the APRR2 transcription factor is causative for the qualitative difference between dark and light green rind in both crops. Further analyses establish the link between sequence or expression level variations in the CmAPRR2 gene and pigment content in the rind and flesh of mature melon fruits. A genome-wide association study (GWAS) of young fruit rind color in a panel composed of 177 diverse melon accessions did not result in any significant association, leading to an earlier assumption that multiple genes are involved in shaping the overall phenotypic variation in this trait. Through resequencing of 25 representative accessions and allelism tests between light rind accessions, we show that multiple independent single nucleotide polymorphisms in the CmAPRR2 gene are causative of the light rind phenotype. The multi-haplotypic nature of this gene explains the lack of detection power obtained through genotyping by sequencing-based GWAS and confirms the pivotal role of this gene in shaping fruit color variation in melon. This study demonstrates the power of combining bi- and multi-allelic designs with deep sequencing, to resolve lack of power due to high haplotypic diversity and low allele frequencies. Due to its central role and broad effect on pigment accumulation in fruits, the APRR2 gene is an attractive target for carotenoid bio-fortification of cucurbit crops

Scientific Publication
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