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פותח על ידי קלירמאש פתרונות בע"מ -
The recent association of a DNA betasatellite with Tomato yellow leaf curl virus in Israel – A new threat to tomato production
Year:
2020
Source of publication :
Crop Protection
Authors :
גנון (גלברט), דנה
;
.
חן, לאה
;
.
לוין, אילן
;
.
לפידות, משה
;
.
Volume :
128
Co-Authors:

Tamar Alon, Svetlana Dobrinin

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

During November 2016, tomato yellow leaf curl virus (TYLCV)-resistant F1 hybrid indeterminate tomato plants cv. Diagrama, grown in a commercial greenhouse in Revaya, Israel, expressed severe symptoms of tomato yellow leaf curl disease (TYLCD). Samples were collected from symptomatic tomato plants, and using TYLCV-specific PCR primers, it was confirmed that these plants were indeed infected with TYLCV. Using the universal betasatellite primer pair B01/B02, followed by cloning and sequencing, it was found that the plants were also infected with a betasatellite. The satellite sequence was deposited in NCBI (GeneBank Acc. No. MK456609). Phylogenetic analysis revealed that the closest similarity, with 96% nucleotide identity, was to cotton leaf curl Gezira betasatellite (CLCuGB). Sampling symptomatic tomato plants in major growing areas in Israel revealed that CLCuGB is disseminated throughout the country. To the best of our knowledge, this is the first identification of a betasatellite, associated with TYLCV, in tomato plants in Israel.

Note:
Related Files :
Betasatellite
DNA
Resistance
Solanum lycopersicum
tomato
Tomato yellow leaf curl virus
Ty-1
TYLCV
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1016/j.cropro.2019.104995
Article number:
104995
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
45740
Last updated date:
02/03/2022 17:27
Creation date:
05/01/2020 09:18
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Scientific Publication
The recent association of a DNA betasatellite with Tomato yellow leaf curl virus in Israel – A new threat to tomato production
128

Tamar Alon, Svetlana Dobrinin

The recent association of a DNA betasatellite with Tomato yellow leaf curl virus in Israel – A new threat to tomato production

During November 2016, tomato yellow leaf curl virus (TYLCV)-resistant F1 hybrid indeterminate tomato plants cv. Diagrama, grown in a commercial greenhouse in Revaya, Israel, expressed severe symptoms of tomato yellow leaf curl disease (TYLCD). Samples were collected from symptomatic tomato plants, and using TYLCV-specific PCR primers, it was confirmed that these plants were indeed infected with TYLCV. Using the universal betasatellite primer pair B01/B02, followed by cloning and sequencing, it was found that the plants were also infected with a betasatellite. The satellite sequence was deposited in NCBI (GeneBank Acc. No. MK456609). Phylogenetic analysis revealed that the closest similarity, with 96% nucleotide identity, was to cotton leaf curl Gezira betasatellite (CLCuGB). Sampling symptomatic tomato plants in major growing areas in Israel revealed that CLCuGB is disseminated throughout the country. To the best of our knowledge, this is the first identification of a betasatellite, associated with TYLCV, in tomato plants in Israel.

Scientific Publication
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