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פותח על ידי קלירמאש פתרונות בע"מ -
Levels of the endosymbiont Rickettsia in the whitefly Bemisia tabaci are influenced by the expression of vitellogenin
Year:
2020
Source of publication :
Insect Molecular Biology
Authors :
ברומין, מרינה
;
.
גנאים, מוראד
;
.
לבדב, גלינה
;
.
קונצדלוב, סבטלנה
;
.
Volume :
29
Co-Authors:
Facilitators :
From page:
241
To page:
255
(
Total pages:
15
)
Abstract:

Bacterial endosymbionts play essential roles in the biology of their arthropod hosts by interacting with internal factors in the host. The whitefly Bemisia tabaci is a worldwide agricultural pest and a supervector for more than 100 plant viruses. Like many other arthropods, Be. tabaci harbours a primary endosymbiont, Porteira aleyrodidarum, and an array of secondary endosymbionts that coexist with Portiera inside bacteriocyte cells. Unlike all of the other secondary symbionts that infect Be. tabaci, Rickettsia has been shown to be an exception by infecting insect organs and not colocalizing with Portiera, and has been shown to significantly impact the insect biology and its interactions with the environment. Little is known about the molecular interactions that underlie insect–symbiont interactions in general, and particularly Be. tabaci–Rickettsia interactions. Here we performed transcriptomic analysis and identified vitellogenin as an important protein that influences the levels of Rickettsia in Be. tabaci. Vitellogenin expression levels were lower in whole insects, but higher in midguts of Rickettsia-infected insects. Immunocapture-PCR assay showed interaction between vitellogenin and Rickettsia, whereas silencing of vitellogenin resulted in nearly complete disappearance of Rickettsia from midguts. Altogether, these results suggest that vitellogenin plays an important role in influencing the levels of Rickettsia in Be. tabaci. © 2019 The Royal Entomological Society

Note:
Related Files :
Bemisia tabaci
Bemisia tabaci (=argentifolii)
Endosymbiont
Rickettsia
vitellogenin
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/imb.12629
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
46101
Last updated date:
02/03/2022 17:27
Creation date:
12/02/2020 12:13
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Scientific Publication
Levels of the endosymbiont Rickettsia in the whitefly Bemisia tabaci are influenced by the expression of vitellogenin
29
Levels of the endosymbiont Rickettsia in the whitefly Bemisia tabaci are influenced by the expression of vitellogenin

Bacterial endosymbionts play essential roles in the biology of their arthropod hosts by interacting with internal factors in the host. The whitefly Bemisia tabaci is a worldwide agricultural pest and a supervector for more than 100 plant viruses. Like many other arthropods, Be. tabaci harbours a primary endosymbiont, Porteira aleyrodidarum, and an array of secondary endosymbionts that coexist with Portiera inside bacteriocyte cells. Unlike all of the other secondary symbionts that infect Be. tabaci, Rickettsia has been shown to be an exception by infecting insect organs and not colocalizing with Portiera, and has been shown to significantly impact the insect biology and its interactions with the environment. Little is known about the molecular interactions that underlie insect–symbiont interactions in general, and particularly Be. tabaci–Rickettsia interactions. Here we performed transcriptomic analysis and identified vitellogenin as an important protein that influences the levels of Rickettsia in Be. tabaci. Vitellogenin expression levels were lower in whole insects, but higher in midguts of Rickettsia-infected insects. Immunocapture-PCR assay showed interaction between vitellogenin and Rickettsia, whereas silencing of vitellogenin resulted in nearly complete disappearance of Rickettsia from midguts. Altogether, these results suggest that vitellogenin plays an important role in influencing the levels of Rickettsia in Be. tabaci. © 2019 The Royal Entomological Society

Scientific Publication
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