חיפוש מתקדם
Food Chemistry
Rui Z - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
Huali X - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
Min S - College of Food Science and Engineering, Gansu Agricultural University, Lanzhu 730070, PR China.
Yang B - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
Mina N - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
Yuanyuan Z - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
Haitao L - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
Prusky D - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China; Department of Postharvest Science of Fresh Produce, The Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel. 
Xiaoyan C - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.

T-2 toxin at low concentrations can induce ROS accumulation and modulate host resistance in plants. NOX plays crucial roles in ROS production and is regulated by Ca2+via direct binding to EF-hand motifs. In this study, the effect of EGTA (Ca2+ chelating agent) on the expression and enzymatic activity of NOX, as well as the activities and corresponding gene expressions involved in ROS metabolism and cell membrane integrity, were investigated in treated slices. Results indicated that EGTA treatment significantly affected gene expression and activity of NOX, and reduced ROS accumulation and cell membrane integrity and the enzymatic activities and gene expression involved in ROS metabolism when exposed to treatment. The addition of exogenous Ca2+ restored the initial relative transcript abundance, ROS accumulation and their activities. Results suggest that Ca2+ affected by EGTA plays a crucial role in NOX activity regulation, ultimately affecting ROS metabolism in slices induced by T-2 toxin.

פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
אודות
תנאי שימוש
Mechanism of Ca<sup>2+</sup>-mediated NOX modulated in ROS metabolism induced by T-2 toxin in potato tuber
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Rui Z - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
Huali X - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
Min S - College of Food Science and Engineering, Gansu Agricultural University, Lanzhu 730070, PR China.
Yang B - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
Mina N - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
Yuanyuan Z - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China.
Haitao L - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
Prusky D - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou 730070, PR China; Department of Postharvest Science of Fresh Produce, The Volcani Center, Agricultural Research Organization, Bet Dagan 50250, Israel. 
Xiaoyan C - College of Science, Gansu Agricultural University, Lanzhou 730070, PR China.
Mechanism of Ca<sup>2+</sup>-mediated NOX modulated in ROS metabolism induced by T-2 toxin in potato tuber

T-2 toxin at low concentrations can induce ROS accumulation and modulate host resistance in plants. NOX plays crucial roles in ROS production and is regulated by Ca2+via direct binding to EF-hand motifs. In this study, the effect of EGTA (Ca2+ chelating agent) on the expression and enzymatic activity of NOX, as well as the activities and corresponding gene expressions involved in ROS metabolism and cell membrane integrity, were investigated in treated slices. Results indicated that EGTA treatment significantly affected gene expression and activity of NOX, and reduced ROS accumulation and cell membrane integrity and the enzymatic activities and gene expression involved in ROS metabolism when exposed to treatment. The addition of exogenous Ca2+ restored the initial relative transcript abundance, ROS accumulation and their activities. Results suggest that Ca2+ affected by EGTA plays a crucial role in NOX activity regulation, ultimately affecting ROS metabolism in slices induced by T-2 toxin.

Scientific Publication
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