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פותח על ידי קלירמאש פתרונות בע"מ -
New Insight Into Pathogenicity and Secondary Metabolism of the Plant Pathogen Penicillium expansum Through Deletion of the Epigenetic Reader SntB
Year:
2020
Source of publication :
Frontiers in Microbiology
Authors :
ברדה, עומר
;
.
סיונוב, אדוארד
;
.
פרוסקי, דב
;
.
Volume :
Co-Authors:

Tannous, J., Department of Medical Microbiology and Immunology, University of Wisconsin – Madison, Madison, WI, United States;
Luciano-Rosario, D., Department of Plant Pathology, University of Wisconsin – Madison, Madison, WI, United States;
Prusky, D.B., Institute of Postharvest and Food Sciences, The Volcani Center, Agricultural Research Organization, Rishon LeZion, Israel, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; 
Keller, N.P., Department of Medical Microbiology and Immunology, University of Wisconsin – Madison, Madison, WI, United States, Food Research Institute, University of Wisconsin – Madison, Madison, WI, United States, Department of Bacteriology, University of Wisconsin – Madison, Madison, WI, United States

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Penicillium expansum is one of the most harmful post-harvest pathogens of pomaceous fruits and the causal agent of blue rot disease. During infection, P. expansum produces the toxic secondary metabolites patulin and citrinin that can impact virulence and, further, render the fruit inedible. Several studies have shown that epigenetic machinery controls synthesis of secondary metabolites in fungi. In this regard, the epigenetic reader, SntB, has been reported to govern the production of multiple toxins in Aspergillus species, and impact virulence of plant pathogenic fungi. Here we show that deletion of sntB in P. expansum results in several phenotypic changes in the fungus including stunted vegetative growth, reduced conidiation, but enhanced germination rates as well as decreased virulence on Golden Delicious apples. In addition, a decrease in both patulin and citrinin biosynthesis in vitro and patulin in apples, was observed. SntB positively regulates expression of three global regulators of virulence and secondary metabolism (LaeA, CreA, and PacC) which may explain in part some of the phenotypic and virulence defects of the PeΔsntB strain. Lastly, results from this study revealed that the controlled environmental factors (low temperatures and high CO2 levels) to which P. expansum is commonly exposed during fruit storage, resulted in a significant reduction of sntB expression and consequent patulin and citrinin reduction. These data identify the epigenetic reader SntB as critical factor regulated in post-harvest pathogens under storage conditions and a potential target to control fungal colonization and decaying of stored fruit.

Note:
Related Files :
citrinin
epigenetic reader
epigenetic regulation
patulin
Penicillium expansum
Secondary metabolism
SntB
virulence
עוד תגיות
תוכן קשור
More details
DOI :
10.3389/fmicb.2020.00610
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
47444
Last updated date:
02/03/2022 17:27
Creation date:
03/05/2020 17:09
You may also be interested in
Scientific Publication
New Insight Into Pathogenicity and Secondary Metabolism of the Plant Pathogen Penicillium expansum Through Deletion of the Epigenetic Reader SntB

Tannous, J., Department of Medical Microbiology and Immunology, University of Wisconsin – Madison, Madison, WI, United States;
Luciano-Rosario, D., Department of Plant Pathology, University of Wisconsin – Madison, Madison, WI, United States;
Prusky, D.B., Institute of Postharvest and Food Sciences, The Volcani Center, Agricultural Research Organization, Rishon LeZion, Israel, College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; 
Keller, N.P., Department of Medical Microbiology and Immunology, University of Wisconsin – Madison, Madison, WI, United States, Food Research Institute, University of Wisconsin – Madison, Madison, WI, United States, Department of Bacteriology, University of Wisconsin – Madison, Madison, WI, United States

New Insight Into Pathogenicity and Secondary Metabolism of the Plant Pathogen Penicillium expansum Through Deletion of the Epigenetic Reader SntB .

Penicillium expansum is one of the most harmful post-harvest pathogens of pomaceous fruits and the causal agent of blue rot disease. During infection, P. expansum produces the toxic secondary metabolites patulin and citrinin that can impact virulence and, further, render the fruit inedible. Several studies have shown that epigenetic machinery controls synthesis of secondary metabolites in fungi. In this regard, the epigenetic reader, SntB, has been reported to govern the production of multiple toxins in Aspergillus species, and impact virulence of plant pathogenic fungi. Here we show that deletion of sntB in P. expansum results in several phenotypic changes in the fungus including stunted vegetative growth, reduced conidiation, but enhanced germination rates as well as decreased virulence on Golden Delicious apples. In addition, a decrease in both patulin and citrinin biosynthesis in vitro and patulin in apples, was observed. SntB positively regulates expression of three global regulators of virulence and secondary metabolism (LaeA, CreA, and PacC) which may explain in part some of the phenotypic and virulence defects of the PeΔsntB strain. Lastly, results from this study revealed that the controlled environmental factors (low temperatures and high CO2 levels) to which P. expansum is commonly exposed during fruit storage, resulted in a significant reduction of sntB expression and consequent patulin and citrinin reduction. These data identify the epigenetic reader SntB as critical factor regulated in post-harvest pathogens under storage conditions and a potential target to control fungal colonization and decaying of stored fruit.

Scientific Publication
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