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פותח על ידי קלירמאש פתרונות בע"מ -
Tomato T2 ribonuclease LE is involved in the response to pathogens
Year:
2020
Source of publication :
Molecular Plant Pathology
Authors :
לרס, אמנון
;
.
סינג, נווין קומאר
;
.
קוטשר, יערית
;
.
ראובני, משה
;
.
Volume :
Co-Authors:

Einat Paz  -  Department of Postharvest Science, Volcani Center, Agricultural Research Organization, Rishon LeZion, Israel; The Robert H. Smith Faculty of Agricultural, Food and Environment Sciences, Hebrew University of Jerusalem, Rehovot, Israel. 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

T2 ribonucleases (RNases) are RNA-degrading enzymes that function in various cellular processes, mostly via RNA metabolism. T2 RNase-encoding genes have been identified in various organisms, from bacteria to mammals, and are most diverse in plants. The existence of T2 RNase genes in almost every organism suggests an important biological function that has been conserved through evolution. In plants, T2 RNases are suggested to be involved in phosphate scavenging and recycling, and are implicated in defence responses to pathogens. We investigated the function of the tomato T2 RNase LE, known to be induced by phosphate deficiency and wounding. The possible involvement of LE in pathogen responses was examined. Expression analysis showed LE induction during fungal infection and by stimuli known to be associated with pathogen inoculation, including oxalic acid and hydrogen peroxide. Analysis of LE-suppressed transgenic tomato lines revealed higher susceptibility to oxalic acid, a cell death-inducing factor, compared to the wild type. This elevated sensitivity of LE-suppressed lines was evidenced by visual signs of necrosis, and increased ion leakage and reactive oxygen species levels, indicating acceleration of cell death. Challenge of the LE-suppressed lines with the necrotrophic pathogen Botrytis cinerea resulted in accelerated development of disease symptoms compared to the wild type, associated with suppressed expression of pathogenesis-related marker genes. The results suggest a role for plant endogenous T2 RNases in antifungal activity .

Note:
Related Files :
Botrytis cinerea
pathogenesis
RNase LE
T2 ribonuclease
tomato
עוד תגיות
תוכן קשור
More details
DOI :
10.1111/mpp.12928
Article number:
0
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
47561
Last updated date:
02/03/2022 17:27
Creation date:
07/05/2020 19:07
You may also be interested in
Scientific Publication
Tomato T2 ribonuclease LE is involved in the response to pathogens

Einat Paz  -  Department of Postharvest Science, Volcani Center, Agricultural Research Organization, Rishon LeZion, Israel; The Robert H. Smith Faculty of Agricultural, Food and Environment Sciences, Hebrew University of Jerusalem, Rehovot, Israel. 

Tomato T2 ribonuclease LE is involved in the response to pathogens

T2 ribonucleases (RNases) are RNA-degrading enzymes that function in various cellular processes, mostly via RNA metabolism. T2 RNase-encoding genes have been identified in various organisms, from bacteria to mammals, and are most diverse in plants. The existence of T2 RNase genes in almost every organism suggests an important biological function that has been conserved through evolution. In plants, T2 RNases are suggested to be involved in phosphate scavenging and recycling, and are implicated in defence responses to pathogens. We investigated the function of the tomato T2 RNase LE, known to be induced by phosphate deficiency and wounding. The possible involvement of LE in pathogen responses was examined. Expression analysis showed LE induction during fungal infection and by stimuli known to be associated with pathogen inoculation, including oxalic acid and hydrogen peroxide. Analysis of LE-suppressed transgenic tomato lines revealed higher susceptibility to oxalic acid, a cell death-inducing factor, compared to the wild type. This elevated sensitivity of LE-suppressed lines was evidenced by visual signs of necrosis, and increased ion leakage and reactive oxygen species levels, indicating acceleration of cell death. Challenge of the LE-suppressed lines with the necrotrophic pathogen Botrytis cinerea resulted in accelerated development of disease symptoms compared to the wild type, associated with suppressed expression of pathogenesis-related marker genes. The results suggest a role for plant endogenous T2 RNases in antifungal activity .

Scientific Publication
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