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פותח על ידי קלירמאש פתרונות בע"מ -
The Potential Risk of Plant-Virus Disease Initiation by Infected Tomatoes
Year:
2020
Source of publication :
צמחים
Authors :
בלאוסוב, אדוארד
;
.
בקלמן, ילנה
;
.
גל-און, עמית
;
.
דומברובסקי, אביב
;
.
לוריא, נטע
;
.
לכמן, עודד
;
.
סמית', אלישבע
;
.
קלפ, חן
;
.
Volume :
9
Co-Authors:

Orly Laskar  - Israel Institute for Biological Research (IIBR), Ness-Ziona 74100, Israel.

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

During 2019, tomato fruits showing viral-like symptoms of marbled yellow spots were abundant in Israel. The new symptoms were distinctive from those typical of tomato brown rugose fruit virus (ToBRFV) infection but resembled symptoms of pepino mosaic virus (PepMV) infection. RT-PCR analysis and the serological tests (enzyme linked immunosorbent assay, western blot and in situ immunofluorescence) revealed and confirmed the presence of both the tobamovirus ToBRFV and the potexvirus PepMV in the symptomatic fruits. A mixture of rod-like and filamentous particles, characteristic of viruses belonging to tobamovirus and potexvirus genera, was visualized by transmission electron microscopy of the tomato fruit viral extract. Sanger sequencing of amplified PepMV-coat protein gene segments showed ~98% sequence identity to the Chilean (CH2)-strain. In a biological assay testing the contribution of traded infected tomatoes to the establishment of tomato plant disease, we applied direct and indirect inoculation modes using Tm-22-resistant tomato plants. The results, assessed by disease symptom development along with serological and molecular analyses, showed that the ToBRFV and PepMV co-infected fruits were an effective inoculum source for disease spread only when fruits were damaged. Importantly, intact fruits did not spread the viral disease. These results added a new factor to disease epidemiology of these viruses.

Note:
Related Files :
Mechanical transmission
Pepino mosaic virus
primary viral inoculum
tomato brown rugose fruit virus
עוד תגיות
תוכן קשור
More details
DOI :
10.3390/plants9050623
Article number:
0
Affiliations:
Database:
PubMed
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
47972
Last updated date:
02/03/2022 17:27
Creation date:
31/05/2020 16:23
You may also be interested in
Scientific Publication
The Potential Risk of Plant-Virus Disease Initiation by Infected Tomatoes
9

Orly Laskar  - Israel Institute for Biological Research (IIBR), Ness-Ziona 74100, Israel.

The Potential Risk of Plant-Virus Disease Initiation by Infected Tomatoes

During 2019, tomato fruits showing viral-like symptoms of marbled yellow spots were abundant in Israel. The new symptoms were distinctive from those typical of tomato brown rugose fruit virus (ToBRFV) infection but resembled symptoms of pepino mosaic virus (PepMV) infection. RT-PCR analysis and the serological tests (enzyme linked immunosorbent assay, western blot and in situ immunofluorescence) revealed and confirmed the presence of both the tobamovirus ToBRFV and the potexvirus PepMV in the symptomatic fruits. A mixture of rod-like and filamentous particles, characteristic of viruses belonging to tobamovirus and potexvirus genera, was visualized by transmission electron microscopy of the tomato fruit viral extract. Sanger sequencing of amplified PepMV-coat protein gene segments showed ~98% sequence identity to the Chilean (CH2)-strain. In a biological assay testing the contribution of traded infected tomatoes to the establishment of tomato plant disease, we applied direct and indirect inoculation modes using Tm-22-resistant tomato plants. The results, assessed by disease symptom development along with serological and molecular analyses, showed that the ToBRFV and PepMV co-infected fruits were an effective inoculum source for disease spread only when fruits were damaged. Importantly, intact fruits did not spread the viral disease. These results added a new factor to disease epidemiology of these viruses.

Scientific Publication
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