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פותח על ידי קלירמאש פתרונות בע"מ -
Development of a point-of-care technology for bacterial identification in milk
Year:
2020
Source of publication :
Talanta
Authors :
אפריאט, ראובן
;
.
ילצוב, יבגני
;
.
צ'לופוביץ, דניאל
;
.
Volume :
219
Co-Authors:
Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

Rapid and sensitive methods for the detection of pathogenic microorganisms are required to monitor contamination in milk and assure public health. A colorimetric antibody-based stack pad system (biosensor) was developed to detect microbiological content of milk based on nitrocellulose membranes. This modification of the lateral flow immunoassay uses a multiple-membrane biosensor containing (from top to bottom): a sample pad, a conjugation pad (with anti-Escherichia coli antibodies conjugated to horseradish peroxidase), six Escherichia coli strain DH5α bacteria-blocking layers and an absorption pad with dry substrate. Different milk samples were tested, with and without DH5α bacteria. This multistep diagnostic assay was up to 1000-fold more sensitive than the standard laboratory ELISA. This is the first step in the development of a simple and portable device for milk-safety monitoring during all processing stages, with the aim of ensuring fresh and healthy milk for consumption.

Note:
Related Files :
Antibodies
Bioassay
Biosensors
colorimetry
Escherichia coli
immunoassay
milk
Pathogenic microorganisms
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.talanta.2020.121223
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
48253
Last updated date:
02/03/2022 17:27
Creation date:
14/06/2020 17:24
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Scientific Publication
Development of a point-of-care technology for bacterial identification in milk
219
Development of a point-of-care technology for bacterial identification in milk

Rapid and sensitive methods for the detection of pathogenic microorganisms are required to monitor contamination in milk and assure public health. A colorimetric antibody-based stack pad system (biosensor) was developed to detect microbiological content of milk based on nitrocellulose membranes. This modification of the lateral flow immunoassay uses a multiple-membrane biosensor containing (from top to bottom): a sample pad, a conjugation pad (with anti-Escherichia coli antibodies conjugated to horseradish peroxidase), six Escherichia coli strain DH5α bacteria-blocking layers and an absorption pad with dry substrate. Different milk samples were tested, with and without DH5α bacteria. This multistep diagnostic assay was up to 1000-fold more sensitive than the standard laboratory ELISA. This is the first step in the development of a simple and portable device for milk-safety monitoring during all processing stages, with the aim of ensuring fresh and healthy milk for consumption.

Scientific Publication
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