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פותח על ידי קלירמאש פתרונות בע"מ -
Physiological response of mammary glands to Escherichia coli infection:A conflict between glucose need for milk production and immune response
Year:
2020
Source of publication :
Scientific Reports
Authors :
יעקובי, שמאי
;
.
מרין, עוזי
;
.
סילניקוב, ניסים
;
.
Volume :
10
Co-Authors:

Shlomo E. Blum - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel.
Oleg Krifuks  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.
Gabriel Leitner - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.

Dan E. Heller - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel

 

Yaniv Lavon - Israel Cattle Breeders Association, Caesarea, 38900, Israel.

 

Facilitators :
From page:
0
To page:
0
(
Total pages:
1
)
Abstract:

The mammary immune and physiological responses to distinct mammary-pathogenic E. coli (MPEC) strains were studied. One gland in each of ten cows were challenged intra-mammary and milk composition (lactose, fat, total protein, casein), biochemical (glucose, glucose-6-phosphate (Glu6P), oxalate, malate, lactate, pyruvate and citrate, malate and lactate dehydrogenases, lactate dehydrogenase (LDH), nitrite, lactic peroxidase, catalase, albumin, lactoferrin, immunoglobulin) and clotting parameters were followed for 35 days post-challenge. Challenge lead to clinical acute mastitis, with peak bacterial counts in milk at 16–24 h post-challenge. Biochemical and clotting parameters in milk reported were partially in accord with lipopolysaccharide-induced mastitis, but increased Glu6P and LDH activity and prolonged lactate dehydrogenase and Glu6P/Glu alterations were found. Some alterations measured in milk resolved within days after challenge, while others endured for above one month, regardless of bacterial clearance, and some reflected physiological responses to mastitis such as the balance between aerobic and anaerobic metabolism (citrate to lactate ratios). The results suggest that E. coli mastitis can be divided into two stages: an acute, clinical phase, as an immediate response to bacterial infection in the mammary gland, and a chronic phase, independent of bacteria clearance, in response to tissue damage caused during the acute phase.

Note:
Related Files :
Acute inflammation
Biochemistry
chronic inflammation
Inflammation
עוד תגיות
תוכן קשור
More details
DOI :
10.1038/s41598-020-66612-7
Article number:
0
Affiliations:
Database:
גוגל סקולר
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
48348
Last updated date:
02/03/2022 17:27
Creation date:
21/06/2020 16:55
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Scientific Publication
Physiological response of mammary glands to Escherichia coli infection:A conflict between glucose need for milk production and immune response
10

Shlomo E. Blum - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel.
Oleg Krifuks  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.
Gabriel Leitner - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.

Dan E. Heller - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel

 

Yaniv Lavon - Israel Cattle Breeders Association, Caesarea, 38900, Israel.

 

Physiological response of mammary glands to Escherichia coli infection:A conflict between glucose need for milk production and immune response

The mammary immune and physiological responses to distinct mammary-pathogenic E. coli (MPEC) strains were studied. One gland in each of ten cows were challenged intra-mammary and milk composition (lactose, fat, total protein, casein), biochemical (glucose, glucose-6-phosphate (Glu6P), oxalate, malate, lactate, pyruvate and citrate, malate and lactate dehydrogenases, lactate dehydrogenase (LDH), nitrite, lactic peroxidase, catalase, albumin, lactoferrin, immunoglobulin) and clotting parameters were followed for 35 days post-challenge. Challenge lead to clinical acute mastitis, with peak bacterial counts in milk at 16–24 h post-challenge. Biochemical and clotting parameters in milk reported were partially in accord with lipopolysaccharide-induced mastitis, but increased Glu6P and LDH activity and prolonged lactate dehydrogenase and Glu6P/Glu alterations were found. Some alterations measured in milk resolved within days after challenge, while others endured for above one month, regardless of bacterial clearance, and some reflected physiological responses to mastitis such as the balance between aerobic and anaerobic metabolism (citrate to lactate ratios). The results suggest that E. coli mastitis can be divided into two stages: an acute, clinical phase, as an immediate response to bacterial infection in the mammary gland, and a chronic phase, independent of bacteria clearance, in response to tissue damage caused during the acute phase.

Scientific Publication
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