חיפוש מתקדם
Scientific Reports

Shlomo E. Blum - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel.
Oleg Krifuks  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.
Gabriel Leitner - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.

Dan E. Heller - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel

 

Yaniv Lavon - Israel Cattle Breeders Association, Caesarea, 38900, Israel.

 

The mammary immune and physiological responses to distinct mammary-pathogenic E. coli (MPEC) strains were studied. One gland in each of ten cows were challenged intra-mammary and milk composition (lactose, fat, total protein, casein), biochemical (glucose, glucose-6-phosphate (Glu6P), oxalate, malate, lactate, pyruvate and citrate, malate and lactate dehydrogenases, lactate dehydrogenase (LDH), nitrite, lactic peroxidase, catalase, albumin, lactoferrin, immunoglobulin) and clotting parameters were followed for 35 days post-challenge. Challenge lead to clinical acute mastitis, with peak bacterial counts in milk at 16–24 h post-challenge. Biochemical and clotting parameters in milk reported were partially in accord with lipopolysaccharide-induced mastitis, but increased Glu6P and LDH activity and prolonged lactate dehydrogenase and Glu6P/Glu alterations were found. Some alterations measured in milk resolved within days after challenge, while others endured for above one month, regardless of bacterial clearance, and some reflected physiological responses to mastitis such as the balance between aerobic and anaerobic metabolism (citrate to lactate ratios). The results suggest that E. coli mastitis can be divided into two stages: an acute, clinical phase, as an immediate response to bacterial infection in the mammary gland, and a chronic phase, independent of bacteria clearance, in response to tissue damage caused during the acute phase.

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הספר "אוצר וולקני"
אודות
תנאי שימוש
Physiological response of mammary glands to Escherichia coli infection:A conflict between glucose need for milk production and immune response
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Shlomo E. Blum - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel; Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel.
Oleg Krifuks  - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.
Gabriel Leitner - National Mastitis Reference Center, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, P.O. Box 6, Bet Dagan, 50250, Israel.

Dan E. Heller - Department of Animal Sciences, Robert H. Smith Faculty of Agriculture, Food and Environment, The Hebrew University of Jerusalem, P.O.Box 12, Rehovot, 76100, Israel

 

Yaniv Lavon - Israel Cattle Breeders Association, Caesarea, 38900, Israel.

 

Physiological response of mammary glands to Escherichia coli infection:A conflict between glucose need for milk production and immune response

The mammary immune and physiological responses to distinct mammary-pathogenic E. coli (MPEC) strains were studied. One gland in each of ten cows were challenged intra-mammary and milk composition (lactose, fat, total protein, casein), biochemical (glucose, glucose-6-phosphate (Glu6P), oxalate, malate, lactate, pyruvate and citrate, malate and lactate dehydrogenases, lactate dehydrogenase (LDH), nitrite, lactic peroxidase, catalase, albumin, lactoferrin, immunoglobulin) and clotting parameters were followed for 35 days post-challenge. Challenge lead to clinical acute mastitis, with peak bacterial counts in milk at 16–24 h post-challenge. Biochemical and clotting parameters in milk reported were partially in accord with lipopolysaccharide-induced mastitis, but increased Glu6P and LDH activity and prolonged lactate dehydrogenase and Glu6P/Glu alterations were found. Some alterations measured in milk resolved within days after challenge, while others endured for above one month, regardless of bacterial clearance, and some reflected physiological responses to mastitis such as the balance between aerobic and anaerobic metabolism (citrate to lactate ratios). The results suggest that E. coli mastitis can be divided into two stages: an acute, clinical phase, as an immediate response to bacterial infection in the mammary gland, and a chronic phase, independent of bacteria clearance, in response to tissue damage caused during the acute phase.

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