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פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of terpene synthase genes potentially involved in black fig fly (Silba adipata) interactions with Ficus carica
Year:
2020
Source of publication :
Plant Science
Authors :
אבדאח, מוופק
;
.
יאהיה, מוסעב
;
.
כבהא, אנאס
;
.
נוואדה, בגווט
;
.
Volume :
298
Co-Authors:

Shaltiel-Harpaz, L. - Tel Hai College, Upper Galilee, 12210, Israel; Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel
Kedoshim, R. - Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel  
Bosamia, T.C. - ICAR-Directorate of Groundnut Research, P.O. Box 362001, Junagadh, Gujarat, India.

Facilitators :
From page:
0
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0
(
Total pages:
1
)
Abstract:

The black fig fly (Silba adipata) is one of the major pests of figs worldwide. This study investigated the effect of pollination on black fig fly infestation and volatile emission during fruit development of facultative parthenocarpic Ficus carica. The results from in-field oviposition preference of black fig fly, olfactory analysis, and fruit volatile profiles indicate that the black fig fly gave a strong preference to unpollinated figs that showed higher emissions of volatile organic compounds. Terpenes are known to be important compounds determining many insect-plant interactions, so we report a transcriptome-based identification and functional characterization of a terpene synthase (TPS) gene family in F. carica. The protein expression in Escherichia coli of eight terpene synthases (TPSs) revealed that three were monoterpene synthases belonging to the TPS-b clade, with FcTPS6 catalyzing the formation of 1,8-cineole while the other two converted GPP into linalool. Four sesquiterpene synthases from the TPS-a clade catalyze the formation of germacrene D (FcTPS1), E-β-caryophyllene (FcTPS2), cadinene (FcTPS3) and δ-elemene (FcTPS5) while one sesquiterpene synthase FcTPS4 from the TPS-b clade showed nerolidol synthase activity. Most of the enzymatic products closely matched the volatile terpenes emitted from fig fruits and all the genes were expressed during fruit development. This study provides new insights into fig-insect interactions and understanding the molecular mechanisms of terpene biosynthesis and could provide the foundations for sustainable pest management strategies.

Note:
Related Files :
Black fig fly
Ficus carica
Fig
Insect-plant interactions
oviposition
pollination
terpene synthase
Volatiles
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.plantsci.2020.110549
Article number:
0
Affiliations:
Database:
סקופוס
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
48502
Last updated date:
02/03/2022 17:27
Creation date:
29/06/2020 16:39
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Scientific Publication
Characterization of terpene synthase genes potentially involved in black fig fly (Silba adipata) interactions with Ficus carica
298

Shaltiel-Harpaz, L. - Tel Hai College, Upper Galilee, 12210, Israel; Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel
Kedoshim, R. - Migal Galilee Research Institute, P.O. Box 831, Kiryat Shmona, 11016, Israel  
Bosamia, T.C. - ICAR-Directorate of Groundnut Research, P.O. Box 362001, Junagadh, Gujarat, India.

Characterization of terpene synthase genes potentially involved in black fig fly (Silba adipata) interactions with Ficus carica

The black fig fly (Silba adipata) is one of the major pests of figs worldwide. This study investigated the effect of pollination on black fig fly infestation and volatile emission during fruit development of facultative parthenocarpic Ficus carica. The results from in-field oviposition preference of black fig fly, olfactory analysis, and fruit volatile profiles indicate that the black fig fly gave a strong preference to unpollinated figs that showed higher emissions of volatile organic compounds. Terpenes are known to be important compounds determining many insect-plant interactions, so we report a transcriptome-based identification and functional characterization of a terpene synthase (TPS) gene family in F. carica. The protein expression in Escherichia coli of eight terpene synthases (TPSs) revealed that three were monoterpene synthases belonging to the TPS-b clade, with FcTPS6 catalyzing the formation of 1,8-cineole while the other two converted GPP into linalool. Four sesquiterpene synthases from the TPS-a clade catalyze the formation of germacrene D (FcTPS1), E-β-caryophyllene (FcTPS2), cadinene (FcTPS3) and δ-elemene (FcTPS5) while one sesquiterpene synthase FcTPS4 from the TPS-b clade showed nerolidol synthase activity. Most of the enzymatic products closely matched the volatile terpenes emitted from fig fruits and all the genes were expressed during fruit development. This study provides new insights into fig-insect interactions and understanding the molecular mechanisms of terpene biosynthesis and could provide the foundations for sustainable pest management strategies.

Scientific Publication
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