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פותח על ידי קלירמאש פתרונות בע"מ -
Influence of tannase from Serratia marcescens strain IMBL5 on enhancing antioxidant properties of green tea
Year:
2020
Authors :
Volume :
27
Co-Authors:

Thiyonila, B. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India.
Kannan, M. - Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Research Centre-Derech HaMaccabim 68, P.O.B 15159, Rishon, LeZion 7505101, Israel.
Paulin Reneeta, N. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India.
Ramya, T. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India.
Kayalvizhi, N. - Department of Zoology, School of Life Sciences, Periyar University, Salem, Tamil Nadu 636 011, India.  
Krishnan, M. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India; Vice-chancellor, Madurai Kamaraj University, Madurai, Tamil Nadu 625021, India

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Abstract:

The present study was focused on the isolation and screening of potential tannase producing gut microbes from dung beetle Onthophagus babirussa IMBL, further optimized and characterized for industrial applications. Serratia marcescens strain IMBL5 was found to be the most potent tannase producer out of 36 isolates. The adjusted process parameters for maximum tannase yield were pH 4.5 at 45 °C and 96 h incubation. The tannase production was increased 4.46 fold i.e. 120.13 U/mL, by varying the concentration of media components using Response surface methodology. The molecular weight of purified tannase was resolved at ~40 kDa through SDS-PAGE analysis. Several functional groups including carboxyl, hydroxyl and amide groups were detected in FT-IR analysis whilst the occurrence of epicatechin gallate peaks detected from HPLC chromatogram signifying the tannin degradation. Tannase also potentially induce the antioxidant properties of green tea extract which was determined through in-vitro analysis. There was no hemolytic activity exhibited in hemolysis assay using human red blood cells which corroborated that tannase derived from S. marcescens strain IMBL5 might be a safe and promising agent in food industry.

Note:
Related Files :
Bacterial tannase
Dung beetle gut microbes
Gel-filtration chromatography
Green tea catechins
Response surface methodology (RSM)
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.bcab.2020.101675
Article number:
0
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סקופוס
Publication Type:
מאמר
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Language:
אנגלית
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ID:
48671
Last updated date:
02/03/2022 17:27
Creation date:
13/07/2020 17:38
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Scientific Publication
Influence of tannase from Serratia marcescens strain IMBL5 on enhancing antioxidant properties of green tea
27

Thiyonila, B. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India.
Kannan, M. - Department of Entomology, Institute of Plant Protection, Agricultural Research Organization, Volcani Research Centre-Derech HaMaccabim 68, P.O.B 15159, Rishon, LeZion 7505101, Israel.
Paulin Reneeta, N. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India.
Ramya, T. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India.
Kayalvizhi, N. - Department of Zoology, School of Life Sciences, Periyar University, Salem, Tamil Nadu 636 011, India.  
Krishnan, M. - Insect Molecular Biology Laboratory, Department of Environmental Biotechnology, Bharathidasan University, Tiruchirapalli, Tamil Nadu 620 024, India; Vice-chancellor, Madurai Kamaraj University, Madurai, Tamil Nadu 625021, India

Influence of tannase from Serratia marcescens strain IMBL5 on enhancing antioxidant properties of green tea

The present study was focused on the isolation and screening of potential tannase producing gut microbes from dung beetle Onthophagus babirussa IMBL, further optimized and characterized for industrial applications. Serratia marcescens strain IMBL5 was found to be the most potent tannase producer out of 36 isolates. The adjusted process parameters for maximum tannase yield were pH 4.5 at 45 °C and 96 h incubation. The tannase production was increased 4.46 fold i.e. 120.13 U/mL, by varying the concentration of media components using Response surface methodology. The molecular weight of purified tannase was resolved at ~40 kDa through SDS-PAGE analysis. Several functional groups including carboxyl, hydroxyl and amide groups were detected in FT-IR analysis whilst the occurrence of epicatechin gallate peaks detected from HPLC chromatogram signifying the tannin degradation. Tannase also potentially induce the antioxidant properties of green tea extract which was determined through in-vitro analysis. There was no hemolytic activity exhibited in hemolysis assay using human red blood cells which corroborated that tannase derived from S. marcescens strain IMBL5 might be a safe and promising agent in food industry.

Scientific Publication
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