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Journal of Applied Microbiology

Wei, J. - , College of Plant Protection, Gansu Agricultural University, Lanzhou, China; College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China
Bi, Y. - , College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
Xue, H. -  College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.

Wang, Y. - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
Zong, Y. - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China
Prusky, D. - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Beit Dagan, Israel

Aims

In this study, the antifungal effect of cinnamaldehyde against Fusarium sambucinum and its underlying mechanisms were determined.

Methods and Results

Minimum inhibitory concentration and minimal fungicidal concentration of cinnamaldehyde were 3 and 4 mmol l−1 on spore germination and colony development assays in vitro , respectively. Furthermore, the lesion diameter of potato tubers and tuber slices inoculated with F. sambucinum was reduced by 76·9 and 69% after treatment with 4 mmol l−1 cinnamaldehyde. Cytometric analyses revelled that cinnamaldehyde significantly affected the integrity of cell membrane firstly, then decreased mitochondrial membrane potential and induced the accumulation of intracellular reactive oxygen species. Meanwhile, high‐performance liquid chromatography results indicated that 3 mmol l−1 cinnamaldehyde could reduce the ergosterol content by 67·94%. This effect was accompanied by a down‐regulation of ERG11, ERG6 and ERG4 which were involved in ergosterol biosynthesis.

Conclusion

Theses results suggest that cinnamaldehyde exerts strong antifungal activity against F. sambucinum , probably by affecting the ergosterol biosynthetic processes what leads to the disruption of cell membrane integrity.

Significance and Impact of the Study

Cinnamaldehyde is a predominant constituent and key flavour compound of cinnamon essential oil. It has been used as a food additive and flavorant. It is expected to be a novel and safe fungicide for controlling dry rot in potato tubes.

פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
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תנאי שימוש
Antifungal activity of cinnamaldehyde against Fusarium sambucinum involves inhibition of ergosterol biosynthesis

Wei, J. - , College of Plant Protection, Gansu Agricultural University, Lanzhou, China; College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China
Bi, Y. - , College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
Xue, H. -  College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.

Wang, Y. - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China.
Zong, Y. - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China
Prusky, D. - College of Food Science and Engineering, Gansu Agricultural University, Lanzhou, China; Department of Postharvest Science of Fresh Produce, Agricultural Research Organization, The Volcani Center, Beit Dagan, Israel

Antifungal activity of cinnamaldehyde against Fusarium sambucinum involves inhibition of ergosterol biosynthesis

Aims

In this study, the antifungal effect of cinnamaldehyde against Fusarium sambucinum and its underlying mechanisms were determined.

Methods and Results

Minimum inhibitory concentration and minimal fungicidal concentration of cinnamaldehyde were 3 and 4 mmol l−1 on spore germination and colony development assays in vitro , respectively. Furthermore, the lesion diameter of potato tubers and tuber slices inoculated with F. sambucinum was reduced by 76·9 and 69% after treatment with 4 mmol l−1 cinnamaldehyde. Cytometric analyses revelled that cinnamaldehyde significantly affected the integrity of cell membrane firstly, then decreased mitochondrial membrane potential and induced the accumulation of intracellular reactive oxygen species. Meanwhile, high‐performance liquid chromatography results indicated that 3 mmol l−1 cinnamaldehyde could reduce the ergosterol content by 67·94%. This effect was accompanied by a down‐regulation of ERG11, ERG6 and ERG4 which were involved in ergosterol biosynthesis.

Conclusion

Theses results suggest that cinnamaldehyde exerts strong antifungal activity against F. sambucinum , probably by affecting the ergosterol biosynthetic processes what leads to the disruption of cell membrane integrity.

Significance and Impact of the Study

Cinnamaldehyde is a predominant constituent and key flavour compound of cinnamon essential oil. It has been used as a food additive and flavorant. It is expected to be a novel and safe fungicide for controlling dry rot in potato tubes.

Scientific Publication
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