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Annals of Applied Biology

Amnon Koren - Hishtil nurseries Ltd Nehalim, Israel.
Nadav Pass - Hishtil nurseries Ltd Nehalim, Israel.

Cucumber green mottle mosaic virus (CGMMV) assigned to the genus Tobamovirus is considered a major disease cause of cucurbits worldwide. A primary route for CGMMV disease spread is via mechanical contact. The virus is highly stable and adheres to various agricultural equipment. In the current study, we examined means to inactivate the virus and reduce disease spread via planting equipment and supplies using various chemicals. We have found that incubations of CGMMV‐infected cucumber plant extracts with MENNO‐Florades 2%, Virocid 3% or Green Up D 20% inactivated the virus and prevented disease spread in a biological assay. Stabilized chlorine formulation (KlorBac), which has the active ingredient troclosene‐sodium (sodium dichloroisocyanurate, SDIC) at 2,000 ppm, was efficient in disinfecting CGMMV contaminated grafting knives in two seconds. Similarly, immersing virus contaminated grafting knives for two seconds in 20% (w/v) non‐fat milk powder reduced infectivity of the contaminated knives. CGMMV contaminated nursery sowing trays could constitute a primary infectious viral source transmitted via irrigation water. CGMMV‐contaminated sowing‐trays immersed in KlorBac 2,000 ppm or active oxygen (Huwa‐San TR‐50) 1%, were efficiently disinfected. Interestingly, hydrophobic insulation of the CGMMV contaminated trays using dry silicone layers reduced initiation of the viral primary infection in CGMMV contaminated new sowing trays but was less efficient in CGMMV contaminated re‐used trays. Importantly, Septadine (0.5% chlorhexidine gluconate) was not effective in disinfection of grafting knives. Notably, CGMMV‐infected cucumber plant extract incubated with 20% (w/v) non‐fat milk powder was refractory to the milk suggesting that virus release from surfaces did not necessarily involve virus inactivation.

פותח על ידי קלירמאש פתרונות בע"מ -
הספר "אוצר וולקני"
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תנאי שימוש
Paths of cucumber green mottle mosaic virus disease spread and disinfectant based management

Amnon Koren - Hishtil nurseries Ltd Nehalim, Israel.
Nadav Pass - Hishtil nurseries Ltd Nehalim, Israel.

Paths of cucumber green mottle mosaic virus disease spread and disinfectant based management

Cucumber green mottle mosaic virus (CGMMV) assigned to the genus Tobamovirus is considered a major disease cause of cucurbits worldwide. A primary route for CGMMV disease spread is via mechanical contact. The virus is highly stable and adheres to various agricultural equipment. In the current study, we examined means to inactivate the virus and reduce disease spread via planting equipment and supplies using various chemicals. We have found that incubations of CGMMV‐infected cucumber plant extracts with MENNO‐Florades 2%, Virocid 3% or Green Up D 20% inactivated the virus and prevented disease spread in a biological assay. Stabilized chlorine formulation (KlorBac), which has the active ingredient troclosene‐sodium (sodium dichloroisocyanurate, SDIC) at 2,000 ppm, was efficient in disinfecting CGMMV contaminated grafting knives in two seconds. Similarly, immersing virus contaminated grafting knives for two seconds in 20% (w/v) non‐fat milk powder reduced infectivity of the contaminated knives. CGMMV contaminated nursery sowing trays could constitute a primary infectious viral source transmitted via irrigation water. CGMMV‐contaminated sowing‐trays immersed in KlorBac 2,000 ppm or active oxygen (Huwa‐San TR‐50) 1%, were efficiently disinfected. Interestingly, hydrophobic insulation of the CGMMV contaminated trays using dry silicone layers reduced initiation of the viral primary infection in CGMMV contaminated new sowing trays but was less efficient in CGMMV contaminated re‐used trays. Importantly, Septadine (0.5% chlorhexidine gluconate) was not effective in disinfection of grafting knives. Notably, CGMMV‐infected cucumber plant extract incubated with 20% (w/v) non‐fat milk powder was refractory to the milk suggesting that virus release from surfaces did not necessarily involve virus inactivation.

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