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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Purification, Properties, and Serology of Carnation Yellow Fleck Virus
Year:
1976
Source of publication :
Phytopathology
Authors :
בר-יוסף, משה
;
.
סמוקלר, מיכאל
;
.
Volume :
66
Co-Authors:
Facilitators :
From page:
835
To page:
838
(
Total pages:
4
)
Abstract:

Carnation yellow fleck virus (CYFV) was partially purified from spray carnations, Dianthus caryophyllus, by clarification with bentonite, concentration with 4% polyethylene glycol, and further purification by rate zonal and isopyonic gradient centrifugation. These methods enabled almost complete removal of carnation mottle virus (CarMV) particles present in the plant material. The purified particles had an unusual ultraviolet absorption spectrum (A260/280 = 1.53), contained about 5% RNA (estimated from a buoyant density p = 1.325 in CsCl), with an extinction coefficient of E 0.1%260=2.27. The molecular weight of the coat protein subunit was about 23,500. In the serological ring precipitin test, CYFV antiserum had a titer of 1/256 and 1/8 against dissociated virus (CYFV-D) in the gel diffusion tests. The antisera did not react with extracts from healthy plants nor with those from plants infected with CarMV, carnation etched ring, and carnation vein mottle viruses.

 

Note:
Related Files :
Carnation Yellow Fleck Virus
Dianthus
plant diseases and disorders
plant protection
viruses and viroids
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/Phyto-66-835
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
49656
Last updated date:
02/03/2022 17:27
Creation date:
07/09/2020 11:30
Scientific Publication
Purification, Properties, and Serology of Carnation Yellow Fleck Virus
66
Purification, Properties, and Serology of Carnation Yellow Fleck Virus

Carnation yellow fleck virus (CYFV) was partially purified from spray carnations, Dianthus caryophyllus, by clarification with bentonite, concentration with 4% polyethylene glycol, and further purification by rate zonal and isopyonic gradient centrifugation. These methods enabled almost complete removal of carnation mottle virus (CarMV) particles present in the plant material. The purified particles had an unusual ultraviolet absorption spectrum (A260/280 = 1.53), contained about 5% RNA (estimated from a buoyant density p = 1.325 in CsCl), with an extinction coefficient of E 0.1%260=2.27. The molecular weight of the coat protein subunit was about 23,500. In the serological ring precipitin test, CYFV antiserum had a titer of 1/256 and 1/8 against dissociated virus (CYFV-D) in the gel diffusion tests. The antisera did not react with extracts from healthy plants nor with those from plants infected with CarMV, carnation etched ring, and carnation vein mottle viruses.

 

Scientific Publication
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