The dissociation reactions of four plant viruses: citrus tristeza virus (CTV), carnation mottle virus (CarMV), carnation yellow fleck virus (CYFV), and tobacco mosaic virus (TMV) and their respective γ-globulin alkaline phosphatase conjugates, sandwiched to antibody microplates, were examined. Treatment with 0.2 M glycine-HCl buffer pH 2.2 for 60 min caused the double antibody sandwiches of CTV and CarMV to dissociate from the antibody-coated microplates. In similar treatments CYFV was eluted less efficiently. The TMV double-sandwich remained undissociated by acidification but it could be partially dissociated under alkaline conditions (pH 12.1). The application of microplate recycling for economizing routine large-scale screening of CTV is described.
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