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אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Reuse of Coated Enzyme-Linked Immunosorbent Assay Plates
Year:
1979
Source of publication :
Phytopathology
Authors :
בר-יוסף, משה
;
.
מוסקוביץ, מירה
;
.
שרפי, יהודית
;
.
Volume :
69
Co-Authors:
Facilitators :
From page:
424
To page:
426
(
Total pages:
3
)
Abstract:

The dissociation reactions of four plant viruses: citrus tristeza virus (CTV), carnation mottle virus (CarMV), carnation yellow fleck virus (CYFV), and tobacco mosaic virus (TMV) and their respective γ-globulin alkaline phosphatase conjugates, sandwiched to antibody microplates, were examined. Treatment with 0.2 M glycine-HCl buffer pH 2.2 for 60 min caused the double antibody sandwiches of CTV and CarMV to dissociate from the antibody-coated microplates. In similar treatments CYFV was eluted less efficiently. The TMV double-sandwich remained undissociated by acidification but it could be partially dissociated under alkaline conditions (pH 12.1). The application of microplate recycling for economizing routine large-scale screening of CTV is described.

Note:
Related Files :
Citrus tristeza virus
Enzyme-Linked Immunosorbent Assay
Immunosorbent assay
Plant Viruses
Tobacco mosaic virus
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/Phyto-69-424
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
49672
Last updated date:
02/03/2022 17:27
Creation date:
07/09/2020 13:51
Scientific Publication
Reuse of Coated Enzyme-Linked Immunosorbent Assay Plates
69
Reuse of Coated Enzyme-Linked Immunosorbent Assay Plates

The dissociation reactions of four plant viruses: citrus tristeza virus (CTV), carnation mottle virus (CarMV), carnation yellow fleck virus (CYFV), and tobacco mosaic virus (TMV) and their respective γ-globulin alkaline phosphatase conjugates, sandwiched to antibody microplates, were examined. Treatment with 0.2 M glycine-HCl buffer pH 2.2 for 60 min caused the double antibody sandwiches of CTV and CarMV to dissociate from the antibody-coated microplates. In similar treatments CYFV was eluted less efficiently. The TMV double-sandwich remained undissociated by acidification but it could be partially dissociated under alkaline conditions (pH 12.1). The application of microplate recycling for economizing routine large-scale screening of CTV is described.

Scientific Publication
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