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פותח על ידי קלירמאש פתרונות בע"מ -
Molecular Cloning of Complementary DNA Sequences of Citrus Tristeza Virus RNA
Year:
1983
Source of publication :
Journal of General Virology
Authors :
בר-יוסף, משה
;
.
רוזנר, אריה
;
.
Volume :
64
Co-Authors:

Ginzburg, Irith - Department of Neurobiology, The Weizmann Institute of Science, Rehovot 76100, Israel

Facilitators :
From page:
1757
To page:
1763
(
Total pages:
7
)
Abstract:

Complementary DNA (cDNA) of citrus tristeza virus (CTV) RNA, synthesized using calf thymus DNA random primers, was converted to a double-stranded form and inserted into the PstI site of the Escherichia coli pBR322 plasmid by the G-C tailing method. Bacterial clones harbouring virus-specific sequences were detected by colony hybridization with a 32P-labelled viral RNA probe. Hybridization patterns of denatured virus RNA revealed the presence of three types of specific clones: those hybridizing with a distinct narrow band corresponding to the full-length virus RNA, those hybridizing with a broader band of virus RNA sequences, and those hybridizing with several distinct virus-related RNA bands. Similar patterns were obtained when these clones were hybridized to purified double-stranded RNA from CTV-infected plants. None of these cDNA clones hybridized with similarly treated preparations extracted from healthy plants. The origin of variation among the CTV clones is discussed.

Note:
Related Files :
cDNA
Citrus tristeza virus
DsRNA
molecular cloning
RNA
Tristeza
viruses and viroids
עוד תגיות
תוכן קשור
More details
DOI :
10.1099/0022-1317-64-8-1757
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
49703
Last updated date:
02/03/2022 17:27
Creation date:
08/09/2020 08:22
Scientific Publication
Molecular Cloning of Complementary DNA Sequences of Citrus Tristeza Virus RNA
64

Ginzburg, Irith - Department of Neurobiology, The Weizmann Institute of Science, Rehovot 76100, Israel

Molecular Cloning of Complementary DNA Sequences of Citrus Tristeza Virus RNA

Complementary DNA (cDNA) of citrus tristeza virus (CTV) RNA, synthesized using calf thymus DNA random primers, was converted to a double-stranded form and inserted into the PstI site of the Escherichia coli pBR322 plasmid by the G-C tailing method. Bacterial clones harbouring virus-specific sequences were detected by colony hybridization with a 32P-labelled viral RNA probe. Hybridization patterns of denatured virus RNA revealed the presence of three types of specific clones: those hybridizing with a distinct narrow band corresponding to the full-length virus RNA, those hybridizing with a broader band of virus RNA sequences, and those hybridizing with several distinct virus-related RNA bands. Similar patterns were obtained when these clones were hybridized to purified double-stranded RNA from CTV-infected plants. None of these cDNA clones hybridized with similarly treated preparations extracted from healthy plants. The origin of variation among the CTV clones is discussed.

Scientific Publication
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