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פותח על ידי קלירמאש פתרונות בע"מ -
Differential Hybridization with Cloned cDNA Sequences for Detecting a Specific Isolate of Citrus Tristeza Virus
Year:
1986
Source of publication :
Phytopathology
Authors :
בר-יוסף, משה
;
.
רוזנר, אריה
;
.
Volume :
76
Co-Authors:

R.F. Lee - University of Florida, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, Lake Alfred 33850

Facilitators :
From page:
820
To page:
824
(
Total pages:
5
)
Abstract:

Two plasmids  with cDNA inserts representing different regions of the citrus tristeza virus (CTV) genome were used to compare five biologically distinct isolates of CTV. One of the plasmids (D2)  hybridized strongly with all of the isolates. The other (D1) plasmid hybridized strongly with four of the isolates. A fifth isolate, which caused severe symptoms, hybridized weakly, if at all, with DI. Similar results were obtained using total plant RNA, RNA extracted from purified and partially purified virus preparations, or dsRNA preparations. Virion RNA of two CTV isolates, HT and VT, used as probes for hybridization with restriction patterns of the D1 CTV clone revealed DNA fragments that carry strain specific sequences.

Note:
Related Files :
cDNA
Citrus tristeza virus
Citrus tristeza virus
Closterovirus
plant diseases and disorders
plant protection
viruses and viroids
עוד תגיות
תוכן קשור
More details
DOI :
10.1094/Phyto-76-820
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
49708
Last updated date:
02/03/2022 17:27
Creation date:
08/09/2020 08:51
Scientific Publication
Differential Hybridization with Cloned cDNA Sequences for Detecting a Specific Isolate of Citrus Tristeza Virus
76

R.F. Lee - University of Florida, Institute of Food and Agricultural Sciences, Citrus Research and Education Center, Lake Alfred 33850

Differential Hybridization with Cloned cDNA Sequences for Detecting a Specific Isolate of Citrus Tristeza Virus

Two plasmids  with cDNA inserts representing different regions of the citrus tristeza virus (CTV) genome were used to compare five biologically distinct isolates of CTV. One of the plasmids (D2)  hybridized strongly with all of the isolates. The other (D1) plasmid hybridized strongly with four of the isolates. A fifth isolate, which caused severe symptoms, hybridized weakly, if at all, with DI. Similar results were obtained using total plant RNA, RNA extracted from purified and partially purified virus preparations, or dsRNA preparations. Virion RNA of two CTV isolates, HT and VT, used as probes for hybridization with restriction patterns of the D1 CTV clone revealed DNA fragments that carry strain specific sequences.

Scientific Publication
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