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קהילה:
אסיף מאגר המחקר החקלאי
פותח על ידי קלירמאש פתרונות בע"מ -
Characterization of the 5′- and 3′-terminal subgenomic RNAs produced by a capillovirus: Evidence for a CP subgenomic RNA
Year:
2009
Source of publication :
Virology
Authors :
בר-יוסף, משה
;
.
Volume :
385
Co-Authors:

Satyanarayana Tatineni - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA and USDA-ARS and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583, USA

Mohammad R. Afunian - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA

Siddarame Gowda - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA

Mark E. Hilf - USDA-ARS, 2001 S. Rock Road, Fort Pierce, FL 34945, USA

William O. Dawson - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA

Facilitators :
From page:
521
To page:
528
(
Total pages:
8
)
Abstract:

The members of Capillovirus genus encode two overlapping open reading frames (ORFs): ORF1 encodes a large polyprotein containing the replication-associated proteins plus a coat protein (CP), and ORF2 encodes a movement protein (MP), located within ORF1 in a different reading frame. Organization of the CP sequence as part of the replicase ORF is unusual in capilloviruses. In this study, we examined the capillovirus genome expression strategy by characterizing viral RNAs produced by Citrus tatter leaf virus (CTLV), isolate ML, a Capillovirus. CTLV-ML produced a genome-length RNA of ∼ 6.5-kb and two 3′-terminal sgRNAs in infected tissue that contain the MP and CP coding sequences (3′-sgRNA1), and the CP coding sequence (3′-sgRNA2), respectively. Both 3′-sgRNAs initiate at a conserved octanucleotide (UUG AAAGA), and are 1826 (3′-sgRNA1) and 869 (3′-sgRNA2) nts with 119 and 15 nt leader sequences, respectively, suggesting that these two 3′-sgRNAs could serve to express the MP and CP. Additionally, accumulation of two 5′-terminal sgRNAs of 5586 (5′-sgRNA1) and 4625 (5′-sgRNA2) nts was observed, and their 3′-termini mapped to 38–44 nts upstream of the transcription start sites of 3′-sgRNAs. The presence of a separate 3′-sgRNA corresponding to the CP coding sequence and its cognate 5′-terminal sgRNA (5′-sgRNA1) suggests that CTLV-ML produces a dedicated sg mRNA for the expression of its CP.

Note:
Related Files :
Citrus tatter leaf virus
Coat protein
Flexiviridae
gene expression
RNA
sgRNAs
viruses and viroids
עוד תגיות
תוכן קשור
More details
DOI :
10.1016/j.virol.2008.12.024
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
49761
Last updated date:
02/03/2022 17:27
Creation date:
09/09/2020 08:25
You may also be interested in
Scientific Publication
Characterization of the 5′- and 3′-terminal subgenomic RNAs produced by a capillovirus: Evidence for a CP subgenomic RNA
385

Satyanarayana Tatineni - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA and USDA-ARS and Department of Plant Pathology, University of Nebraska, Lincoln, NE 68583, USA

Mohammad R. Afunian - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA

Siddarame Gowda - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA

Mark E. Hilf - USDA-ARS, 2001 S. Rock Road, Fort Pierce, FL 34945, USA

William O. Dawson - Citrus Research and Education Center, University of Florida-IFAS, Lake Alfred, FL 33850, USA

Characterization of the 5′- and 3′-terminal subgenomic RNAs produced by a capillovirus: Evidence for a CP subgenomic RNA

The members of Capillovirus genus encode two overlapping open reading frames (ORFs): ORF1 encodes a large polyprotein containing the replication-associated proteins plus a coat protein (CP), and ORF2 encodes a movement protein (MP), located within ORF1 in a different reading frame. Organization of the CP sequence as part of the replicase ORF is unusual in capilloviruses. In this study, we examined the capillovirus genome expression strategy by characterizing viral RNAs produced by Citrus tatter leaf virus (CTLV), isolate ML, a Capillovirus. CTLV-ML produced a genome-length RNA of ∼ 6.5-kb and two 3′-terminal sgRNAs in infected tissue that contain the MP and CP coding sequences (3′-sgRNA1), and the CP coding sequence (3′-sgRNA2), respectively. Both 3′-sgRNAs initiate at a conserved octanucleotide (UUG AAAGA), and are 1826 (3′-sgRNA1) and 869 (3′-sgRNA2) nts with 119 and 15 nt leader sequences, respectively, suggesting that these two 3′-sgRNAs could serve to express the MP and CP. Additionally, accumulation of two 5′-terminal sgRNAs of 5586 (5′-sgRNA1) and 4625 (5′-sgRNA2) nts was observed, and their 3′-termini mapped to 38–44 nts upstream of the transcription start sites of 3′-sgRNAs. The presence of a separate 3′-sgRNA corresponding to the CP coding sequence and its cognate 5′-terminal sgRNA (5′-sgRNA1) suggests that CTLV-ML produces a dedicated sg mRNA for the expression of its CP.

Scientific Publication
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