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פותח על ידי קלירמאש פתרונות בע"מ -
Hen egg yolk as a source of antiviral antibodies in the enzymelinked immunosorbent assay (ELISA): A comparison of two plant viruses
Year:
1980
Source of publication :
Journal of Virological Methods
Authors :
בר-יוסף, משה
;
.
Volume :
1
Co-Authors:

M. Malkinson - Kimron Veterinary Institute, Bet-Dagan, Israel

Facilitators :
From page:
179
To page:
183
(
Total pages:
5
)
Abstract:

A method of raising antibodies against plant viruses in hen egg yolk is described. Laying hens were immunized with citrus tristeza virus (CTV) or tobacco mosaic virus-avocado isolate (TMV-A). Antiviral antibodies in the yolks of sequentially laid eggs as well as in the serum were titrated by the (heterologous) antiglobulin double antibody sandwich form of the enzyme-linked immunosorbent assay (HADAS-ELISA). Antibodies first appeared in yolk 7 days after injection and peak levels were attained on day 9–11; these levels persisted for about 6–12 days. Non-specific yolk antibodies were removed by absorption with an extract of uninfected plant tissue. Using the HADAS-ELISA technique we found that yolk titres were equal to, or higher than those in serum. The benefits of using laying hens over conventional laboratory animals as a source of antiviral antibody are discussed.

Note:
Related Files :
Antibodies
Antiviral
egg yolk
plant diseases and disorders
plant protection
Plant Viruses
viruses and viroids
עוד תגיות
תוכן קשור
More details
DOI :
https://doi.org/10.1016/0166-0934(80)90014-2
Article number:
0
Affiliations:
Database:
Publication Type:
מאמר
;
.
Language:
אנגלית
Editors' remarks:
ID:
49827
Last updated date:
02/03/2022 17:27
Creation date:
09/09/2020 12:30
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Scientific Publication
Hen egg yolk as a source of antiviral antibodies in the enzymelinked immunosorbent assay (ELISA): A comparison of two plant viruses
1

M. Malkinson - Kimron Veterinary Institute, Bet-Dagan, Israel

Hen egg yolk as a source of antiviral antibodies in the enzymelinked immunosorbent assay (ELISA): A comparison of two plant viruses

A method of raising antibodies against plant viruses in hen egg yolk is described. Laying hens were immunized with citrus tristeza virus (CTV) or tobacco mosaic virus-avocado isolate (TMV-A). Antiviral antibodies in the yolks of sequentially laid eggs as well as in the serum were titrated by the (heterologous) antiglobulin double antibody sandwich form of the enzyme-linked immunosorbent assay (HADAS-ELISA). Antibodies first appeared in yolk 7 days after injection and peak levels were attained on day 9–11; these levels persisted for about 6–12 days. Non-specific yolk antibodies were removed by absorption with an extract of uninfected plant tissue. Using the HADAS-ELISA technique we found that yolk titres were equal to, or higher than those in serum. The benefits of using laying hens over conventional laboratory animals as a source of antiviral antibody are discussed.

Scientific Publication
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